Recombinant NEDD8 protein

Aliases: Nedd-8, Neural Precursor Cell Expressed Developmentally Down Regulated 8, Ubiquitin Like Protein NEDD8, Neddylin
Catalog No: 82094 Format: 100 µg ¥3,760 Add to Cart
Catalog No: 82694 Format: 1 mg ¥25,600 Add to Cart

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Expressed In: E. coli Protein Species: Human

Contents

A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.

Background

NEDD8 is a ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis via its conjugation to a limited number of cellular proteins, such as cullins or p53/TP53. Attachment of NEDD8 to cullins is critical for the recruitment of E2 to the cullin-RING-based E3 ubiquitin-protein ligase complex, thus facilitating polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins. Attachment of NEDD8 to p53/ TP53 inhibits p53/TP53 transcriptional activity. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M.

Application Notes

This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product.  All available data for this product is shown. 

Protein Details

Recombinant NEDD8 protein that includes full length of human NEDD8 protein (accession number NP_006147.1) was expressed in E. coli and contains an N-terminal His and FLAG tag with a molecular weight of 12.51kDa. The purity of the protein is ≥ 95% by SDS-PAGE.

 
Recombinant NEDD8 protein Coomassie gel

Recombinant NEDD8 protein

12.5% SDS-PAGE Coomassie staining

MW: 12.51 kDa

Purity: ≥ 95%

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.