Active Motif eBooks

In this eBook, we are inspired by both the original landmark publication “The Hallmarks of Aging” by López-Otín et al., Cell, 2013 and their 2023 update (López-Otín et al., Cell, 2023), which now includes processes ranging from epigenetic alterations to genomic instability, stem cell exhaustion to inflammation, changes in nuclear architecture and other key features of aging at the cellular level. With Active Motif’s expertise in epigenetics, our focus here will be on the aging hallmark of epigenetic changes, with an emphasis on key biological targets and pathways as presented during a series of seminars during Chromatin-Con 2023: Epigenetics of Aging. Several hallmarks are discussed, as well as methods for their analysis featured during this event.

DNA甲基化已被证明是几乎所有生物体内基因表达和基因组组织的一个关键的表观遗传调节方式，因此出现了许多检测5-mC和5-hmC的方法。各种各样的检测方法可能会让人不知该如何选择，且不知道从何开始。然而，虽然有许多研究DNA甲基化的方法，但可以根据特定实验的目标和资源来确定最好的策略，每种方法都有优势和劣势。

这本免费的电子书旨在帮助大家了解常见的检测方法，并帮助研究人员选择适合他们实验的最佳方法。

本书概述了ChIP技术与它在表观遗传学中的应用，详细介绍了ChIP实验中的关键步骤、注意事项、如何确定ChIP是否成功、如何设计ChIP-qPCR引物、样品超声处理等。

让您在读完本指南后，轻松成为ChIP实验好手！

CUT&Tag 是一项非常有价值的技术，可用于研究组蛋白修饰和一些转录因子的基因组定位，可揭示蛋白质与 DNA 之间的相互作用或识别目标蛋白质的 DNA 结合位点。

这本免费电子书概述了 CUT&Tag、以及它与其它基于 ChIP 的protocol的方法的比较，还有开始在研究中使用 CUT&Tag 所需了解的一切。

The chromatin immunoprecipitation (ChIP) assay changed the way researchers can study protein-DNA interactions and epigenetic modifications of histones. However, ChIP assays are difficult. ChIP protocols have many steps that each require optimization, making it hard to consistently generate high-quality data you can trust.

This free eBook makes it easy to become a ChIP assay expert by covering the main steps in the ChIP workflow and giving pointers on how to achieve the best results.

There is increasing evidence that many neurodevelopmental, neurodegenerative, and psychiatric disorders are caused at least in part by aberrant epigenetic modifications.

As a result, neuroscience researchers are now beginning to investigate the epigenetic mechanisms responsible for regulation of gene expression in neuronal cells and how this impacts normal brain function. This free eBook will help you get started with epigenetics and guide you towards the best approaches to take to answer your specific research questions.

MicroRNAs (miRNAs) have been associated with essentially all aspects of cancer, including diagnosis, staging, progression, prognosis, and response to treatment. Their misexpression can contribute to tumor initiation, growth, and metastasis, making miRNAs attractive therapeutic targets and potentially useful diagnostic tools.

The rapid advancements in microRNA (miRNA) research requires new experimental tools to enable scientists to accurately and efficiently study the role of miRNAs in cancer and other diseases. This MicroRNAs in Cancer eBook summarizes the current miRNA research approaches and outlines how to design and execute an miRNA target screen.