A Beginner’s Guide to Performing Chromatin Immunoprecipitation Assays
The chromatin immunoprecipitation (ChIP) assay changed the way researchers can study protein-DNA interactions and epigenetic modifications of histones. However, ChIP assays are difficult. ChIP protocols have many steps that each require optimization, making it hard to consistently generate high-quality data you can trust.
This free eBook makes it easy to become a ChIP assay expert by covering the main steps in the ChIP workflow and giving pointers on how to achieve the best results.
An In-Depth Review of Studying DNA Methylation in Cancer Research
The link between epigenetics and cancer has been demonstrated through the identification of DNA methylation biomarkers associated with many different types of cancer as well as mutations in epigenetic enzymes that contribute to tumorigenesis, metastasis, and cancer progression.
This DNA Methylation and Cancer eBook provides a comprehensive in-depth review of DNA methylation, DNA hydroxymethylation, as well as other DNA methylation variants. The tools available to study DNA methylation in cells or tissue are also discussed.
A Guide to Getting Started with Epigenetics in Neuroscience Research
There is increasing evidence that many neurodevelopmental, neurodegenerative, and psychiatric disorders are caused at least in part by aberrant epigenetic modifications.
As a result, neuroscience researchers are now beginning to investigate the epigenetic mechanisms responsible for regulation of gene expression in neuronal cells and how this impacts normal brain function. This free eBook will help you get started with epigenetics and guide you towards the best approaches to take to answer your specific research questions.
Guide to Studying miRNAs and Performing Luciferase-Based Reporter Screens
MicroRNAs (miRNAs) have been associated with essentially all aspects of cancer, including diagnosis, staging, progression, prognosis, and response to treatment. Their misexpression can contribute to tumor initiation, growth, and metastasis, making miRNAs attractive therapeutic targets and potentially useful diagnostic tools.
The rapid advancements in microRNA (miRNA) research requires new experimental tools to enable scientists to accurately and efficiently study the role of miRNAs in cancer and other diseases. This MicroRNAs in Cancer eBook summarizes the current miRNA research approaches and outlines how to design and execute an miRNA target screen.