Active Motif eBooks

CUT&RUN is an epigenetic method used to investigate genome-wide distribution of chromatin-associated proteins and their modifications. Because it is sensitive, specific, and requires fewer cells than ChIP, it has become a popular new tool for identifying binding patterns of transcription factors or histone modifications genome-wide.

This eBook is a complete resource for CUT&RUN, from the background and novelty of the method, to evaluating when it’s the best choice for your research, including sample data, analysis tips, and resources for further study.

In this eBook, we are inspired by both the original landmark publication “The Hallmarks of Aging” by López-Otín et al., Cell, 2013 and their 2023 update (López-Otín et al., Cell, 2023), which now includes processes ranging from epigenetic alterations to genomic instability, stem cell exhaustion to inflammation, changes in nuclear architecture and other key features of aging at the cellular level. With Active Motif’s expertise in epigenetics, our focus here will be on the aging hallmark of epigenetic changes, with an emphasis on key biological targets and pathways as presented during a series of seminars during Chromatin-Con 2023: Epigenetics of Aging. Several hallmarks are discussed, as well as methods for their analysis featured during this event.

DNA methylation has been shown to be a crucial epigenetic regulator of gene expression and genomic organization in nearly all organisms, leading to the emergence of numerous methods for mapping 5-mC and 5-hmC. The sheer number of options can be overwhelming and make it difficult to know where to start. However, while there are many approaches for studying DNA methylation, the best tactic can be determined based on the goals and resources for a particular experiment, with each method having advantages and drawbacks.

This free eBook is designed to help explain popular methods and to help researchers decide on the best approach for their work.

CUT&Tag is an invaluable method to investigate genomic localization of histone modifications and some transcription factors that reveals interactions between proteins and DNA or identifies DNA binding sites for proteins of interest.

This free eBook provides an overview of CUT&Tag, how it compares to other ChIP-based protocols, and everything you need to know to get started using CUT&Tag in your research.

The chromatin immunoprecipitation (ChIP) assay changed the way researchers can study protein-DNA interactions and epigenetic modifications of histones. However, ChIP assays are difficult. ChIP protocols have many steps that each require optimization, making it hard to consistently generate high-quality data you can trust.

This free eBook makes it easy to become a ChIP assay expert by covering the main steps in the ChIP workflow and giving pointers on how to achieve the best results.

There is increasing evidence that many neurodevelopmental, neurodegenerative, and psychiatric disorders are caused at least in part by aberrant epigenetic modifications.

As a result, neuroscience researchers are now beginning to investigate the epigenetic mechanisms responsible for regulation of gene expression in neuronal cells and how this impacts normal brain function. This free eBook will help you get started with epigenetics and guide you towards the best approaches to take to answer your specific research questions.

MicroRNAs (miRNAs) have been associated with essentially all aspects of cancer, including diagnosis, staging, progression, prognosis, and response to treatment. Their misexpression can contribute to tumor initiation, growth, and metastasis, making miRNAs attractive therapeutic targets and potentially useful diagnostic tools.

The rapid advancements in microRNA (miRNA) research requires new experimental tools to enable scientists to accurately and efficiently study the role of miRNAs in cancer and other diseases. This MicroRNAs in Cancer eBook summarizes the current miRNA research approaches and outlines how to design and execute an miRNA target screen.