Myelin Basic Protein, dephosphorylated

substrate for in vitro kinase assays

Myelin Basic Protein, MBP, is useful as a substrate for in vitro kinase assays, including MAPK, PKA, PKC cyclin-dependent and calmodulin-dependent protein kinases.

Name Format Cat No. Price  
Myelin Basic Protein, dephosphorylated 5 mg 31314 Discontinued

Myelin Basic Protein is purified from bovine brain using SP-Sepharose™ FPLC and dephosphorylated using lambda phosphatase. Lambda phosphatase is then inactivated by the addition of EDTA and heat treatment (65°C for 1 hour). The estimated purity is >95% based on Coomasie staining.

Kinase assay

Figure 1: Use of MBP as a kinase assay substrate.
Myelin Basic Protein was tested in an in vitro kinase assay by incubation with (Lane 1) or without MAPK2 (Lane 2), followed by Western blot detection using an MBP phospho Thr98 antibody. To the right of the Western blot is a lane from a Coomassie-stained SDS-PAGE gel on which 4 µg of purified MBP was loaded.

Contents & Storage

5 mg of MBP (18.5 kDa) at a concentration of 5 mg/ml in 10 mM MOPS (pH 7.0), 0.05% sodium azide, 0.02 µg/ml inactive lambda phosphatase, 0.18 mM MnCl2. Store at -20°C. Upon initial thaw, aliquot the product in small volumes to avoid freeze-thaw cycles. All reagents are guaranteed stable for 6 months when stored properly.