CUT&Tag (Cleavage Under Targets and Tagmentation) utilizes a protein A (pA) Tn5 chimera (pA-Tn5) and a mouse or rabbit primary antibody to a histone or histone mark of interest to tether the pA-Tn5. This directs the Tn5 activity to tagment the DNA around the target of interest genome-wide, without any NGS library preparation steps, to reveal the genetic sequences of those sites of interest. CUT&Tag requires much less input material and sequencing read depths than ChIP-Seq, and can be perfomed in 1-2 days.
Profile histone marks with rabbit or mouse antibodies from as few as 5,000 cells.
Profile histone marks with rabbit or mouse antibodies from 0.5 to 10 mg of tissue.
Pure recombinant pA-Tn5 transposase enzyme in 10 µg, 100 µg or larger quantities.
Multiplex up to 96 CUT&Tag samples.