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CUT&RUN（Cleavage Under Targets & Release Using Nuclease）是一种研究各种染色质相关蛋白及其修饰的全基因组分布的表观遗传学方法，是ChIC（chromatin immunocleavage）的衍生方法。类似于ChIP（chromatin immunoprecipitation），CUT&RUN也是利用抗体靶向染色质相关修饰和蛋白，但其所需细胞量和测序深度比传统ChIP更低。
Active Motif 专注于生产组蛋白、组蛋白修饰、染色质蛋白和其他因子的高质量抗体，其中包括越来越多经我们内部实验验证在 CUT&RUN 检测中效果良好的抗体。
已发表用于CUT&RUN的Active Motif公司生产抗体
以下是已发表的使用这些抗体的应用，仅供参考。Active Motif 公司可能尚未对这些抗体在这些应用中的使用进行验证。
用于CUT&RUN的抗体的已发表文献
下表列出的抗体已经我们内部实验验证在ChIC/CUT&RUN Assay Kit中效果良好，点击抗体名称以获得完整信息。
| Name | Isotype | Reactivity | Application | Format | Cat No. | Price | |
|---|---|---|---|---|---|---|---|
| BRD2 antibody (pAb) | IgG | Human | ChIP-Seq, Chromatin Immunoprecipitation, CUT&RUN | 100 µl | 61797 | ¥5,610 | Add to Cart |
| 10 µl | 61798 | ¥1,380 | Add to Cart | ||||
| BRD3 antibody (pAb) | IgG | Human, Mouse | ChIP-Seq, Chromatin Immunoprecipitation, CUT&RUN, Western blot | 100 µl | 61489 | ¥5,610 | Add to Cart |
| 10 µl | 61490 | ¥1,380 | Add to Cart | ||||
| AbFlex® BRD4 antibody (rAb) | IgG | Human | ChIP-Seq, Chromatin Immunoprecipitation, CUT&RUN | 100 µg | 91301 | ¥4,240 | Add to Cart |
| 10 µg | 91302 | ¥940 | Add to Cart | ||||
| CTCF antibody (pAb) | IgG | Human | ChIP-Seq, Chromatin Immunoprecipitation, CUT&RUN, CUT&Tag, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Supershift, Western blot | 100 µl | 61311 | Discontinued | |
| 10 µl | 61312 | Discontinued | |||||
| 50 µl | 61932 | Discontinued | |||||
| Ets-1 antibody (pAb) | Serum | Human, Mouse | ChIP-Seq, CUT&RUN, Western blot | 200 µl | 39580 | ¥5,610 | Add to Cart |
| 10 µl | 39581 | ¥1,380 | Add to Cart | ||||
| AbFlex® EZH2 antibody (rAb) | IgG2a | Human | CUT&RUN, Immunoprecipitation | 100 µg | 91159 | ¥4,240 | Add to Cart |
| 10 µg | 91160 | ¥940 | Add to Cart | ||||
| EZH2 antibody (pAb) | Serum | Human, Mouse | ChIP-Seq, Chromatin Immunoprecipitation, CUT&RUN, Immunoprecipitation | 100 µl | 39901 | ¥5,610 | Add to Cart |
| 50 µl | 39002 | ¥3,250 | Add to Cart | ||||
| 10 µl | 39902 | ¥1,380 | Add to Cart | ||||
| AbFlex® Histone H3K4me3 antibody (rAb) | IgG2a | Human, Wide Range Predicted | ChIP-Seq, Chromatin Immunoprecipitation, CUT&RUN, CUT&Tag, Dot blot, Western blot | 100 µg | 91263 | ¥4,240 | Add to Cart |
| 10 µg | 91264 | ¥940 | Add to Cart | ||||
| Histone H3K4me3 antibody (pAb) | Serum | Budding Yeast, Human, Mouse, Wide Range Predicted | ChIP-Seq, Chromatin Immunoprecipitation, CUT&RUN, CUT&Tag, Dot Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western blot | 100 µl | 39159 | ¥5,610 | Add to Cart |
| 50 µl | 39060 | ¥3,250 | Add to Cart | ||||
| 10 µl | 39160 | ¥1,380 | Add to Cart | ||||
| Histone H3K4me3 antibody (pAb) | IgG | Budding Yeast, Human, Wide Range Predicted | ChIP-Seq, Chromatin Immunoprecipitation, CUT&RUN, CUT&Tag, Dot Blot, Immunocytochemistry, Immunofluorescence, Western blot | 100 µg | 39915 | ¥5,610 | Add to Cart |
| 50 µg | 39016 | ¥3,250 | Add to Cart | ||||
| 10 µg | 39916 | ¥1,380 | Add to Cart | ||||
