Whether you are performing drug discovery screening, analyzing enzyme kinetics or monitoring changes in histone modifications, substrate selection is critical. Nucleosomes offer the advantage of providing a more biologically relevant substrate when compared to histone proteins alone or synthetic peptides.
HMT activity assay comparing recombinant nucleosomes and histone octamers as substrates.
To best mimic cellular biology with your in vitro assay, use Active Motif’s Recombinant Nucleosomes. Active Motif’s pre-assembled Recombinant Nucleosomes save you time and effort by providing pre-assembled human nucleosomes. Each recombinant nucleosome is comprised of octamers of unmodified core histone proteins (H2A, H2B, H3 and H4) bound by DNA. These intact nucleosomes are ideal substrates for in vitro studies. Nucleosomes are available to study both Histone H3.1 and Histone H3.3 variants. Additionally, choose from unlabeled or biotin-labeled nucleosomes to give you flexibility in your experimental design.
|Name||Expressed In||Format||Cat No.||Price|
|Recombinant Histone Octamer (H3.1)||E. coli||100 µg||31470||¥3,360||Buy|
|Recombinant Histone Octamer (H3.1) - biotinylated||E. coli||50 µg||31471||¥3,600||Buy|
|Recombinant Histone Octamer (H3.3)||E. coli||100 µg||31472||¥3,280||Buy|
|Recombinant Histone Octamer (H3.3) - biotinylated||E. coli||50 µg||31473||¥3,600||Buy|
|Recombinant Mononucleosomes (H1.2)||E. coli||20 µg||81272||¥3,400||Buy|
|Recombinant Mononucleosomes (H1.2) - biotin||E. coli||20 µg||81273||¥3,480||Buy|
|Recombinant Mononucleosomes (H2A.Bbd) - biotinylated||E. coli||20 µg||31556||¥3,320||Buy|
|Recombinant Mononucleosomes (H2A.X)||E. coli||20 µg||81125||¥3,160||Buy|
|Recombinant Mononucleosomes (H2A.X) - biotinylated||E. coli||20 µg||31582||¥3,160||Buy|
|Recombinant Mononucleosomes (H2A.Z)||E. coli||50 µg||81072||¥3,160||Buy|