AVAILABLE PRODUCTS

ChIP-Sequencing（ChIP-Seq）服务

“样本到数据”的ChIP-Seq服务

What our customers are saying about us ...

"Active Motif took the time to listen to our questions, understand our goal and discuss all options and implications. Not only did the ChIP-Seq study confirm our data-set, it also highlighted a novel mechanism. The quality of the data and service was amazing. Excellent science, informed and helpful people and quality data."
David Briere, PhD
Principal Scientist
Mirati Therapeutics
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The ChIP-Seq Service includes:

Customers submit fixed cell pellets or frozen tissues, then we:

  1. Prepare chromatin samples and sonicate.
  2. Perform ChIP with a ChIP-qualified antibody.
  3. Construct ChIP-Seq libraries.
  4. Perform Next-Gen sequencing.
  5. Analyze & deliver the data.

To learn more, please give us a call or send us an Epigenetic Services Information Request. You can also download Active Motif’s Epigenetic Services Profile.

 
Name Cat No. Price  
FactorPath™ ChIP-Seq 25001 Get Quote
HistonePath™ ChIP-Seq 25011 Get Quote
TranscriptionPath™ ChIP-Seq 25031 Get Quote
Input-Seq 25046 Get Quote
Active Motif Epigenetic Services Profile
Epigenetic Services Cell Fixation Protocol
Epigenetic Services Sample Submission Form
Epigenetic Services Citations

Generating data is only half the battle in ChIP-Seq. When the sequencing is complete, tens of millions of short sequence tags must be mapped back to the genome followed by peak calling. Peak calling is complicated by the fact that different algorithms are required for accurate peak calling depending on the antibody used. Thousands to tens of thousands of binding sites must then be exported into a meaningful output that relates the data to genes and allows for multiple samples to be compared to one another. Challenges continue with clustering, heat maps and graphical representations of genome-wide localization patterns. This type of in-depth bioinformatics analysis is beyond the capabilities of most labs. That is why data analysis is part of the standard package for all of our ChIP-Seq Service projects.

A heat map showing the relative occupancy of demethylase KDM1A and Histone H3 dimethyl Lys4 (H3K4me2) at the transcription start sites of all genes bound by these factors
A graph depicting the relative occupancy of demethylase KDM1A and Histone H3 dimethyl Lys4 (H3K4me2) at the transcription start sites of all genes bound by these factors
Figure 3: Compilation of all genomic binding sites relative to gene transcription start sites (TSS).

ChIP-Seq was performed with antibodies against the histone demethylase KDM1A and one of its targets, Histone H3 dimethyl Lys4 (H3K4me2). Thousands of binding sites were identified and the positions of all binding sites, as they relate to gene annotations, were compiled. Left. A heat map was created using all genes bound by H3K4me2 and KDM1A and the occupancy of these factors around the transcription start site (TSS) is depicted. Top. The same data depicted a different way shows KDM1A occupancy between the two peaks of H3K4me2 occupancy.

The selected papers below cite the use of and/or provide additional information about ChIP-Seq Services provided by Active Motif’s Epigenetic Services: