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ChIP-Sequencing(ChIP-Seq)服务

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- 表观遗传学服务
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- ChIP-Sequencing(ChIP-Seq)服务
客户对我们的评价
"Active Motif花时间了解我们的问题,了解了我们的目标并讨论了所有选项和含义。 不仅仅通过ChIP-Seq实验验证了我们的数据,而且还强调了一种新的机理。 数据和服务的质量惊人地好。 优秀的科学知识,知识渊博且乐于助人的人员和优质的数据。"
David Briere, PhD
首席科学家
Mirati Therapeutics
查看完整的客户评价 >
ChIP-Seq服务包括:
客户提供固定的细胞沉淀或冷冻组织,然后我们:
- 准备染色质样品并超声处理。
- 使用验证过的抗体进行ChIP实验。
- 建立ChIP-Seq测序文库。
- 进行二代测序。
- 分析并发送数据。
要了解更多信息,请电话联系我们或向我们发送表观遗传服务信息申请。 您还可以下载Active Motif的表观遗传服务介绍。
Name | Cat No. | Price | |
---|---|---|---|
FactorPath™ ChIP-Seq | 25001 | Get Quote | |
HistonePath™ ChIP-Seq | 25011 | Get Quote | |
TranscriptionPath™ ChIP-Seq | 25031 | Get Quote | |
Input-Seq | 25046 | Get Quote |
在ChIP-Seq中,得到数据只是成功的一半。 测序完成后,必须将数千万个短序列标签比对到基因组,然后进行peak calling 。peak calling非常复杂,根据所使用的抗体,需要不同的算法来进行准确的peak calling。 然后必须将成千上万的结合位点输出到有意义的结果中,将数据与基因相关联,并允许多个样本相互比较。 聚类,热图和全基因组定位模式的可视化呈现仍面临挑战。 这种类型的深入生物信息学分析超出了大多数实验室的能力。 因此,数据分析是我们所有ChIP-Seq服务项目的标准服务的一部分。


图3:相对于基因转录起始位点(TSS)的所有基因组结合位点的汇集。
用组蛋白脱甲基酶KDM1A及其靶标之一的组蛋白H3K4me2抗体进行ChIP-Seq实验。 因为与基因注释有关,鉴定了数千个结合位点,并汇集了所有结合位点的位置。左图。使用由H3K4me2和KDM1A结合的所有基因创建了一个热图,并描述了转录起始位点(TSS)周围这些因子的结合率。上图。相同的数据使用不同方式显示KDM1A 在H3K4me2结合的两个峰之间的结合率。
以下部分论文引用了Active Motif表观遗传服务提供的有关ChIP-Seq服务和/或其他信息:
- “Reduced H3K27me3 and DNA Hypomethylation Are Major Drivers of Gene Expression in K27M Mutant Pediatric High-Grade Gliomas” by Bender et al (2013) Cancer Cell 24(5):660-672.
- “Atrial Identity Is Determined by a COUP-TFII Regulatory Network” by Wu et al (2013) Developmental Cell 25(4):417-426.
- “Specification of type 2 innate lymphocytes by the transcriptional determinant Gfi1” by Spooner et al (2013) Nature Immunology 14(2):1229-1236.
- “EZH2 inhibition as a therapeutic strategy for lymphoma with EZH2-activating mutations” by McCabe et al (2012) Nature 492(7427):108-112.
- “Loss of the Tumor Suppressor BAP1 Causes Myeloid Transformation” by Dey et al (2012) Science 337(6101):1541-1546.
- “ChIP sequencing of cyclin D1 reveals a transcriptional role in chromosomal instability in mice” by Casimiro et al (2011) Journal of Clinical Investigation 122(3):833-843.
- “Global Characterization of Transcriptional Impact of the SRC-3 Coregulator” by Lanz et al (2010) Molecular Endocrinology 24(4):859-872.
- “A ChIP-seq defined genome-wide map of vitamin D receptor binding: Associations with disease and evolution” by Ramagopalan et al (2010) Genome Research 20(10):1352-1360.
- “miR-155 Inhibition Sensitizes CD4+ Th Cells for TREG Mediated Suppression” by Stahl et al (2009) PLoS ONE 4(9):e7158.