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AbFlex® Recombinant Antibodies

defined antibodies for highly specific, reproducible performance

AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. In addition to flexible labeling and purification options, each AbFlex® antibody contains a Sortase recognition motif (LPXTG) to covalently add fluorophores, enzymatic substrates (HRP, AP...etc), peptides, DNA, drugs or other labels to the antibody in a directed and reproducible manner using our Sortag-IT Labeling Kits. Every antibody also contains a 6xHis tag, which can be used with nickel-based purification systems, and an avidin tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA.

Advantages of AbFlex® Recombinant Antibodies (rAbs)

  • Flexible labeling & purification options
  • Consistent performance across lots
  • Highly specific and sensitive antibodies
  • Reliable, long-term supply
  • Animal-free production

Application Data

The following data file includes application data for all of our AbFlex® antibodies and the monoclonal antibodies from which they are based.
Application Data File.

 
 
Name Isotype Reactivity Appl. Format Cat No. Price  
AbFlex® 5-methylcytosine antibody (rAb) IgG2a Other (Not Species Specific) DB 100 µg 91187 ¥5,438 Buy Now
10 µg 91188 ¥1,088 Buy Now
AbFlex® Ago 1/2/3 antibody (rAb) IgG2a Human IP 100 µg 91217 ¥5,438 Buy Now
10 µg 91218 ¥1,088 Buy Now
AbFlex® AM-Tag antibody (rAb) IgG2a Other (Wide Range) ChIP, ELISA, WB 100 µg 91111 ¥5,438 Buy Now
10 µg 91112 ¥1,088 Buy Now
AbFlex® AM-Tag antibody (rAb) (biotin) IgG2a Other (Wide Range) ChIP, ELISA, WB 100 µg 91109 ¥5,873 Buy Now
10 µg 91110 ¥1,233 Buy Now
AbFlex® ATM phospho Ser1981 antibody (rAb) IgG2a Human, Mouse DB, WB 100 µg 91207 ¥5,438 Buy Now
10 µg 91208 ¥1,088 Buy Now
AbFlex® Bcl6 antibody (rAb) IgG2a Human WB 100 µg 91287 ¥5,438 Buy Now
10 µg 91288 ¥1,088 Buy Now
AbFlex® BMI-1 antibody (rAb) IgG2a Human, Mouse ChIP, ChIP-Seq, WB 100 µg 91195 ¥5,438 Buy Now
10 µg 91196 ¥1,088 Buy Now
AbFlex® BRD4 antibody (rAb) IgG Human ChIP, ChIP-Seq 100 µg 91301 ¥5,438 Buy Now
10 µg 91302 ¥1,088 Buy Now
AbFlex® BrdU antibody (rAb) IgG2a Other (Wide Range) IF 100 µg 91269 ¥5,438 Buy Now
10 µg 91270 ¥1,088 Buy Now
AbFlex® Cas9 antibody (rAb) IgG2a Human ELISA, WB 100 µg 91123 ¥5,438 Buy Now
10 µg 91124 ¥1,088 Buy Now
AbFlex® SaCas9 antibody (rAb) IgG2a Other (Not Species Specific) WB 100 µg 91279 ¥5,438 Buy Now
10 µg 91280 ¥1,088 Buy Now
AbFlex® CHD3 antibody (rAb) IgG2a Drosophila IP, WB 100 µg 91273 ¥5,438 Buy Now
10 µg 91274 ¥1,088 Buy Now
AbFlex® CTCF antibody (rAb) IgG Human ChIP-Seq, WB 100 µg 91285 ¥5,438 Buy Now
10 µg 91286 ¥1,088 Buy Now
AbFlex® Dicer antibody (rAb) IgG2a Mouse WB 100 µg 91241 ¥5,438 Buy Now
10 µg 91242 ¥1,088 Buy Now
AbFlex® EED antibody (rAb) IgG2a Human ChIP, ChIP-Seq, ELISA 100 µg 91135 ¥5,438 Buy Now
10 µg 91136 ¥1,088 Buy Now
AbFlex® EHMT2 (G9a) antibody (rAb) IgG2a Human WB 100 µg 91229 ¥5,438 Buy Now
10 µg 91230 ¥1,088 Buy Now
AbFlex® EZH2 antibody (rAb) IgG2a Human IP 100 µg 91159 ¥5,438 Buy Now
10 µg 91160 ¥1,088 Buy Now
AbFlex® HA-Tag antibody (rAb) IgG2a Other (Not Species Specific) IP 100 µg 91271 ¥5,438 Buy Now
10 µg 91272 ¥1,088 Buy Now
AbFlex® HDAC1 antibody (rAb) IgG2a Human, Mouse ChIP-Seq, WB 100 µg 91215 ¥5,438 Buy Now
10 µg 91216 ¥1,088 Buy Now
AbFlex® HDAC2 antibody (rAb) IgG2a Human ChIP-Seq, WB 100 µg 91197 ¥5,438 Buy Now
10 µg 91198 ¥1,088 Buy Now
AbFlex® HDAC3 antibody (rAb) IgG2a Human WB 100 µg 91199 ¥5,438 Buy Now
10 µg 91200 ¥1,088 Buy Now
AbFlex® HIF-1 alpha antibody (rAb) IgG2a Bovine, Human, Mouse, Rat ELISA 100 µg 91139 ¥5,438 Buy Now
10 µg 91140 ¥1,088 Buy Now
AbFlex® His-Tag antibody (rAb) IgG2a Other (Not Species Specific) WB 100 µg 91183 ¥5,438 Buy Now
10 µg 91184 ¥1,088 Buy Now
AbFlex® Histone H3K4me0 antibody (rAb) IgG2a Human, Other (Wide Range) DB, WB 100 µg 91267 ¥5,438 Buy Now
10 µg 91268 ¥1,088 Buy Now
AbFlex® Histone H3K4me1 antibody (rAb) IgG2a Human WB 100 µg 91289 ¥5,438 Buy Now
10 µg 91290 ¥1,088 Buy Now
AbFlex® Histone H3K4me3 antibody (rAb) IgG2a Human, Other (Wide Range) ChIP, ChIP-Seq, DB, WB 100 µg 91263 ¥5,438 Buy Now
10 µg 91264 ¥1,088 Buy Now
AbFlex® Histone H3K9ac antibody (rAb) IgG2a Human, Other (Wide Range) ChIP, ChIP-Seq, ELISA, ICC, IF, WB 100 µg 91103 ¥5,438 Buy Now
10 µg 91104 ¥1,088 Buy Now
AbFlex® Histone H3K9ac antibody (rAb) (biotin) IgG2a Human, Other (Wide Range) ChIP, ChIP-Seq, ELISA, ICC, IF, WB 100 µg 91101 ¥5,873 Buy Now
10 µg 91102 ¥1,233 Buy Now
AbFlex® Histone H3K9me0 antibody (rAb) IgG2a Human, Other (Wide Range) ChIP, ChIP-Seq, ELISA, ICC, IF, WB 100 µg 91155 ¥5,438 Buy Now
10 µg 91156 ¥1,088 Buy Now
AbFlex® Histone H3S10ph antibody (rAb) IgG2a Human DB, WB 100 µg 91131 ¥5,438 Buy Now
10 µg 91132 ¥1,088 Buy Now
AbFlex® Histone H3K27ac antibody (rAb) IgG2a Human ChIP-Seq, ELISA, WB 100 µg 91193 ¥5,438 Buy Now
10 µg 91194 ¥1,088 Buy Now
AbFlex® Histone H3K27me3 antibody (rAb) IgG Human DB, WB 100 µg 91167 ¥5,438 Buy Now
10 µg 91168 ¥1,088 Buy Now
AbFlex® Histone H3K36me3 antibody (rAb) IgG2a Other (Wide Range) ChIP, ChIP-Seq, WB 100 µg 91265 ¥5,438 Buy Now
10 µg 91266 ¥1,088 Buy Now
AbFlex® Histone H3K56ac antibody (rAb) IgG2a Human, Other (Wide Range) ChIP-Seq, ELISA 100 µg 91127 ¥5,438 Buy Now
10 µg 91128 ¥1,088 Buy Now
AbFlex® Histone H3.3 antibody (rAb) IgG2a Human ChIP-Seq, WB 100 µg 91191 ¥5,438 Buy Now
10 µg 91192 ¥1,088 Buy Now
AbFlex® Histone H4K20me1 antibody (rAb) IgG2a Human WB 100 µg 91291 ¥5,438 Buy Now
10 µg 91292 ¥1,088 Buy Now
AbFlex® Histone H4K20me3 antibody (rAb) IgG2a Human, Other (Wide Range) ChIP, ChIP-Seq, ELISA, ICC, IF, WB 100 µg 91107 ¥5,438 Buy Now
10 µg 91108 ¥1,088 Buy Now
AbFlex® Histone H4K20me3 antibody (rAb) (biotin) IgG2a Human, Other (Wide Range) ChIP, ChIP-Seq, ELISA, ICC, IF, WB 100 µg 91105 ¥5,873 Buy Now
10 µg 91106 ¥1,233 Buy Now
AbFlex® JMJD2A antibody (rAb) IgG2a Human ELISA, WB 100 µg 91143 ¥5,438 Buy Now
10 µg 91144 ¥1,088 Buy Now
AbFlex® KDM5A antibody (rAb) IgG2a Human ChIP-Seq, WB 100 µg 91211 ¥5,438 Buy Now
10 µg 91212 ¥1,088 Buy Now
AbFlex® Lamin A/C antibody (rAb) IgG2a Human IF, WB 100 µg 91189 ¥5,438 Buy Now
10 µg 91190 ¥1,088 Buy Now
AbFlex® MAZ antibody (rAb) IgG2a Human WB 100 µg 91205 ¥5,438 Buy Now
10 µg 91206 ¥1,088 Buy Now
AbFlex® MEIS 1/2/3 antibody (rAb)  IgG2a Human, Mouse WB 100 µg 91259 ¥5,438 Buy Now
10 µg 91260 ¥1,088 Buy Now
AbFlex® Mi-2 beta (CHD4) antibody (rAb) IgG2a Human WB 100 µg 91275 ¥5,438 Buy Now
10 µg 91276 ¥1,088 Buy Now
AbFlex® Mili / PiwiL2 antibody (rAb) IgG2a Mouse WB 100 µg 91253 ¥5,438 Buy Now
10 µg 91254 ¥1,088 Buy Now
AbFlex® MITF antibody (rAb) IgG2a Human ChIP-Seq 100 µg 91201 ¥5,438 Buy Now
10 µg 91202 ¥1,088 Buy Now
AbFlex® MPP8 antibody (rAb) IgG2a Human WB 100 µg 91277 ¥5,438 Buy Now
10 µg 91278 ¥1,088 Buy Now
AbFlex® Myc-Tag antibody (rAb) IgG2a Human WB 100 µg 91203 ¥5,438 Buy Now
10 µg 91204 ¥1,088 Buy Now
AbFlex® c-Myc antibody (rAb) IgG2a Human WB 100 µg 91213 ¥5,438 Buy Now
10 µg 91214 ¥1,088 Buy Now
AbFlex® Nanog antibody (rAb) IgG2a Mouse WB 100 µg 91251 ¥5,438 Buy Now
10 µg 91252 ¥1,088 Buy Now
AbFlex® Notch1 antibody (rAb) IgG2a Human WB 100 µg 91243 ¥5,438 Buy Now
10 µg 91244 ¥1,088 Buy Now
AbFlex® Nucleolin antibody (rAb) IgG2a Canine, Human WB 100 µg 91231 ¥5,438 Buy Now
10 µg 91232 ¥1,088 Buy Now
AbFlex® p53 antibody (rAb) IgG2a Human WB 100 µg 91247 ¥5,438 Buy Now
10 µg 91248 ¥1,088 Buy Now
AbFlex® PBX2 antibody (rAb) IgG2a Human, Mouse WB 100 µg 91227 ¥5,438 Buy Now
10 µg 91228 ¥1,088 Buy Now
AbFlex® Pim2 antibody (rAb) IgG2a Human WB 100 µg 91239 ¥5,438 Buy Now
10 µg 91240 ¥1,088 Buy Now
AbFlex® Rad21 antibody (rAb) IgG2a Human, Mouse IF, WB 100 µg 91245 ¥5,438 Buy Now
10 µg 91246 ¥1,088 Buy Now
AbFlex® RNA Pol II antibody (rAb) IgG2a Human ChIP-Seq, IF, WB 100 µg 91151 ¥5,438 Buy Now
10 µg 91152 ¥1,088 Buy Now
AbFlex® RNA Pol II CTD phospho Tyr1 antibody (rAb) IgG2a Human ELISA, IF, WB 100 µg 91219 ¥5,438 Buy Now
10 µg 91220 ¥1,088 Buy Now
AbFlex® RNA pol II CTD phospho Ser2 antibody (rAb) IgG2a Human ChIP, ChIP-Seq, ELISA, ICC, IF, WB 100 µg 91115 ¥5,438 Buy Now
10 µg 91116 ¥1,088 Buy Now
AbFlex® RNA pol II CTD phospho Ser5 antibody (rAb) IgG2a Human ChIP, ChIP-Seq, ELISA, ICC, IF, WB 100 µg 91119 ¥5,438 Buy Now
10 µg 91120 ¥1,088 Buy Now
AbFlex® RNA pol II CTD phospho Ser5 antibody (rAb) (biotin) IgG2a Human ChIP, ChIP-Seq, ELISA, ICC, IF, WB 100 µg 91117 ¥5,873 Buy Now
10 µg 91118 ¥1,233 Buy Now
AbFlex® SMARCA1 / SNF2L1 antibody (rAb) IgG2a Human WB 100 µg 91225 ¥5,438 Buy Now
10 µg 91226 ¥1,088 Buy Now
AbFlex® Snail antibody (rAb) IgG2a Human WB 100 µg 91255 ¥5,438 Buy Now
10 µg 91256 ¥1,088 Buy Now
AbFlex® Suz12 antibody (rAb) IgG2a Human IP 100 µg 91163 ¥5,438 Buy Now
10 µg 91164 ¥1,088 Buy Now
AbFlex® TET1 antibody (rAb) IgG2a Human, Mouse ChIP-Seq 100 µg 91171 ¥5,438 Buy Now
10 µg 91172 ¥1,088 Buy Now
AbFlex® Ubiquitin antibody (rAb) IgG2a Other (Wide Range) WB 100 µg 91257 ¥5,438 Buy Now
10 µg 91258 ¥1,088 Buy Now

AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. The unique advantages of the AbFlex® antibody are its flexible labeling and purification options. Each AbFlex® antibody contains a Sortase recognition motif (LPXTG) to covalently add fluorophores, enzymatic substrates (HRP, AP...etc), peptides, DNA, drugs or other labels to the antibody in a directed and reproducible manner using our Sortag-IT Labeling Kits. Every antibody also contains a 6xHis tag, which can be used with nickel-based purification systems, and an avidin tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA.

AbFlex antibody with flexible labeling and purification options
AbFlex® recombinant antibody (rAb) with flexible labeling and purification options.

AbFlex® antibodies are specifically labeled at the end of the constant region of the heavy chain to avoid interference with antigen recognition and functionality. This is important as it ensures the labeling process maintains the integrity of the antibody so signal is not diminished as a result of non-functional antibodies. In contrast, commonly used chemical labeling methods add labels to the antibody in a random fashion. The randomness of this process has a high potential to block the antigen-binding site and render the antibody ineffective. Chemical labeling can also deposit labels on the Fc region of the antibody which has the potential to obstruct interactions with protein A.

AbFlex® directed labeling versus chemical labeling
Differences in label distribution between AbFlex® direct labeling and chemical labeling.

 

Prior to the development of recombinant antibodies, researchers relied on polyclonal and monoclonal antibodies. Polyclonal antibodies recognize multiple epitopes on the same antigen. This increases antibody affinity, but often has a higher potential for cross-reactivity. Monoclonal antibodies represent a single antibody producing B cell that binds to one unique epitope. However, monoclonal antibodies can be lost or evolve over time.

Unlike monoclonal antibodies, which can experience gene loss, mutations and cell-line drift that alter antibody performance over time and lead to variability across antibody lots, recombinant antibodies are highly consistent because they contain the same immunoglobulin heavy and light chain DNA sequences in every lot. This provides batch-to-batch reproducibility in antibody performance and eliminates the need to re-evaluate or compare different antibody lots, thereby, saving valuable time and effort on assay development.

To ensure optimal performance, each AbFlex® antibody is carefully screened for specificity and sensitivity. Only heavy and light chain sequences that produce highly specific antibodies, as verified in our bead-based binding assay, are selected. Antibodies that utilize the AbFlex® direct labeling approach also show greater sensitivity than their parent monoclonal antibodies across a variety of applications.

Another advantage of AbFlex® recombinant antibodies is the fact that they are produced outside of an animal system. Polyclonal antibodies rely on an animal’s immune response and epitope specificity, both of which are highly variable across animals and immunizations. Polyclonal antibodies are also hindered by having a finite supply, making it difficult to perform long-term antibody projects or reproduce data over time or across labs. With AbFlex® recombinant antibodies, the full-length IgG is expressed in a mammalian system to ensure a consistent, stable antibody supply. Additionally, any ethical or animal welfare concerns associated with antibody production and maintenance are avoided.

The following list contains Active Motif's AbFlex® recombinant antibodies (rAbs). For information about site-specific labeling of the AbFlex® antibodies, please visit our collection of Sortag-IT™ Labeling Kits.