AVAILABLE PRODUCTS

DNA Library Prep Kit for Illumina®

Dual Index NGS Kit for ChIP-Seq, CUT&RUN, and ds methylated DNA assays

 

DNA Library Prep Kit for Illumina® Overview

DNA Library Prep Kit for IlluminaDNA Library Prep Kit for Illumina®
Dual Index Primers Set 1 for for IlluminaDual Index Primers Set 1 for for Illumina®
Dual Index Primers Set 2 for for IlluminaDual Index Primers Set 2 for for Illumina®
❮ ❯
     
 

Related Products:

The DNA Library Prep Kit for Illumina is optimized for double-stranded templates generated in Active Motif’s ChIP-Seq and ChIC/CUT&RUN assay kits. This kit ensures efficient, high-quality library preparation for ChIP-Seq and CUT&RUN applications and is provided in a 48 reaction kit, which along with the Dual Index Primers Set 1 and 2 enable multiplexing up to 96 reactions on an Illumina next-generation sequencing system flow cell.


DNA Library Prep for Illumina Performance

  • Works with 0.5 ng to 10 ng dsDNA
  • Consistent fragment sizes

Recommended for:

  • ChIC/CUT&RUN Assay Kit
  • ChIP-IT High Sensitivity
  • Low Cell ChIP
  • 5hmC Profiling Kit
  • MethylCollector MBD Capture Kit

DNA Library Prep Kit for Illumina Workflow

How the DNA Library Prep Kit Works(Click image to enlarge)

 

DNA Library Prep Kit for Illumina® Contents

 

DNA Library Prep Kit for Illumina (Cat. No. 53220)

  • End Repair Buffer, store at -20°C
  • End Repair Enzyme Mix, store at -20°C
  • Illumina Adapter, store at -20°C
  • T4 DNA Ligase, store at -20°C
  • Q5 Polymerase NGS MM, store at -20°C
  • TT Buffer, store at -20°C
  • SPRI Beads, store at 4°C
  • PEG, store at 4°C

Dual Index Primers Set 1 for Illumina (Cat. No. 53221)

  • AM i5-001 Primer, store at -20°C
  • AM i5-002 Primer, store at -20°C
  • AM i5-003 Primer, store at -20°C
  • AM i5-004 Primer, store at -20°C
  • AM i5-005 Primer, store at -20°C
  • AM i5-006 Primer, store at -20°C
  • AM i7-001 Primer, store at -20°C
  • AM i7-002 Primer, store at -20°C
  • AM i7-003 Primer, store at -20°C
  • AM i7-004 Primer, store at -20°C
  • AM i7-005 Primer, store at -20°C
  • AM i7-006 Primer, store at -20°C
  • AM i7-007 Primer, store at -20°C
  • AM i7-008 Primer, store at -20°C

Dual Index Primers Set 2 for Illumina (Cat. No. 53222)

  • AM i5-007 Primer, store at -20°C
  • AM i5-008 Primer, store at -20°C
  • AM i5-009 Primer, store at -20°C
  • AM i5-010 Primer, store at -20°C
  • AM i5-011 Primer, store at -20°C
  • AM i5-012 Primer, store at -20°C
  • AM i7-001 Primer, store at -20°C
  • AM i7-002 Primer, store at -20°C
  • AM i7-003 Primer, store at -20°C
  • AM i7-004 Primer, store at -20°C
  • AM i7-005 Primer, store at -20°C
  • AM i7-006 Primer, store at -20°C
  • AM i7-007 Primer, store at -20°C
  • AM i7-008 Primer, store at -20°C
 

DNA Library Prep Kit for Illumina® Data

Quality Libraries Generated for CTCF CUT&RUN
(Click image to enlarge)

Figure 1. Quality Libraries Generated for CTCF CUT&RUN with DNA Library Prep Kit for Illumina
IGV Browser tracks shown for 500,000 K562 cells assayed in triplicate with ChIC/CUT&RUN Assay Kit (Cat. No. 53180) targeting CTCF (Cat. No. 91285). Libraries were generated using DNA Library Prep Kit for Illumina (Cat. No. 53220) or a Competitor’s kit, and compared with ENCODE ChIP data for CTCF.

 

Quality Libraries Generated for IGV Browser tracks(Click image to enlarge)

Figure 2. Quality Libraries Generated for H3K4me3 CUT&RUN with DNA Library Prep Kit for Illumina
IGV Browser tracks shown for 500,000 K562 cells assayed in triplicate with ChIC/CUT&RUN Assay Kit (Cat. No. 53180) targeting H3K4me3 (Cat. No. 39060). Libraries were generated using DNA Library Prep Kit for Illumina (Cat. No. 53220) or a Competitor’s kit, and compared with ENCODE ChIP data for H3K4me3.

 

Heat Map of Pearson’s Correlation
(Click image to enlarge)

Figure 3. Heat Map of Pearson’s Correlation Between DNA Library Prep Kit for Illumina and Competitor’s Kit.
Triplicate CUT&RUN samples targeting H3K4me3 show strong correlation with the results generated with a Competitor’s Kit.

 

Quality ChIP-Seq Data(Click image to enlarge)

Figure 4. Quality ChIP-Seq Data Generated with DNA Library Prep Kit for Illumina
IGV browser tracks shown for triplicate ChIP reactions targeting CTCF with ChIP-IT High Sensitivity (Cat. No. 53040) and DNA Library Prep Kit for Illumina or a Competitor’s library prep kit.

 

DNA Library Prep Kit comparison(Click image to enlarge)

Figure 5. DNA Library Prep Kit for Illumina Works With 0.5 ng to 10 ng dsDNA
0.5, 1, and 10 ng E. coli DNA were sequenced after libraries were prepared with DNA Library Prep Kit for Illumina and results were comparable to a Competitor’s Kit.

 

DNA Library Prep Kit for Illumina® Documents

 

 

You might also be interested in:

 
Name Format Cat No. Price  
DNA Library Prep Kit for Illumina® 48 rxns 53220 ¥14,760 Add to Cart
Dual Index Primers Set 1 for Illumina® 48 rxns 53221 ¥1,370 Add to Cart
Dual Index Primers Set 2 for Illumina® 48 rxns 53222 ¥1,370 Add to Cart
DNA Library Prep Kit for Illumina Manual [English]
53221 and 53222 Dual Index Primers Set 1 and 2 Sequence Information
Dual Index Primer Set 1 for Illumina TDS
Dual Index Primer Set 2 for Illumina TDS