RNA Rol II

RNA pol II - going, going, paused...

RNA-polymerase II (Pol II)-mediated transcription is a highly regulated process that determines cellular function and cell identity through the synthesis of mRNAs. RNA Pol II regulation at the stage of initiation is a key mechanism to control gene expression, so that a thorough understanding of gene regulation includes the actual process of transcription and this key driver of the process - RNA Pol II initiation, pausing, and active elongation.

Active Motif offers a unique suite of antibodies designed to identify RNA Pol II, and those C-terminal domains (CTDs) whose phosphorylation states are critical for enzyme activity. Another important approach is to measure RNA Pol II occupancy across the genome using ChIP, which provides information on start site selection, pre-initiation complex (PIC) formation, pausing, and transcription rates. Our TranscriptionPath™ Assay is uniquely suited for this type of analysis.

Antibodies List

Product Application Format Cat. No.
AbFlex® RNA Pol II antibody (rAb) ChIP-Seq, IF, WB 10 µg 91152
100 µg 91151
RNA pol II antibody (mAb) ChIP, ChIP-Seq, ICC, IF, WB 50 µl 39497
200 µl 39097
RNA pol II antibody (mAb) WB 10 µg 61082
100 µg 61081
RNA pol II antibody (mAb) ChIP, ChIP-Seq, ICC, IF, WB 10 µg 61668
100 µg 61667
AbFlex® RNA Pol II CTD phospho Tyr1 antibody (rAb) ELISA, IF, WB 10 µg 91220
100 µg 91219
RNA Pol II CTD phospho Tyr1 antibody (mAb) ChIP, IP, WB 10 µg 61384
100 µg 61383
AbFlex® RNA pol II CTD phospho Ser2 antibody (rAb) ChIP, ChIP-Seq, ELISA, ICC, IF, WB 10 µg 91116
100 µg 91115
RNA pol II CTD phospho Ser2 antibody (mAb) ChIP, ChIP-Seq, ICC, IF, IP, WB 10 µg 61084
100 µg 61083
RNA pol II CTD phospho Ser2 antibody (mAb) ICC, IF, WB 10 µg 61700
100 µg 61699
RNA pol II CTD phospho Ser2 antibody (pAb) IP, WB 10 µl 39564
200 µl 39563
RNA pol II CTD Ser2ph / Ser5ph antibody (mAb) ChIP, ChIP-Seq, ICC, IF, WB 10 µg 61666
100 µg 61665
RNA Pol II CTD phospho Thr4 antibody (mAb) ChIP, DB, ICC, IF, IP, WB 10 µg 61362
100 µg 61361
RNA Pol II CTD phospho Thr4 antibody (pAb) WB 10 µg 61308
100 µg 61307
AbFlex® RNA pol II CTD phospho Ser5 antibody (rAb) ChIP, ChIP-Seq, ELISA, ICC, IF, WB 10 µg 91120
100 µg 91119
RNA pol II CTD phospho Ser5 antibody (mAb) ChIP, ChIP-Seq, ICC, IF, IP, WB 10 µg 61086
100 µ 61085
RNA pol II CTD phospho Ser5 antibody (mAb) ICC, IF, WB 10 µg 61702
100 µg 61701
RNA pol II CTD phospho Ser5 antibody (pAb) ChIP, ICC, IF, WB 10 µl 39234
100 µl 39233
RNA pol II CTD phospho Ser5 antibody (pAb) ChIP, ChIP-Seq, WB 10 µg 39750
100 µg 39749
RNA pol II CTD phospho Ser7 antibody (mAb) ChIP, ChIP-Seq, ICC, IF, IP, WB 10 µg 61088
100 µg 61087
RNA pol II CTD phospho Ser7 antibody (mAb) ICC, IF, WB 10 µg 61704
100 µg 61703

 

TranscriptionPath

TranscriptionPath assay provides an alternate approach to RNA-based gene expression methods. TranscriptionPath is a Chromatin Immunoprecipitation-based assay used to measure transcription rates as a function of RNA Polymerase II occupancy across the genome. Unlike other RNA-based techniques, the method enables you to measure transcription rates without the influence of RNA half-life, and with the added advantage of using the same material that is used in your transcription factor targeted ChIP experiments. So now you can correlate transcription factor binding with transcriptional changes in the same experiment.

  • Measure transcription rates without the influence of RNA half-life.
  • Ideal for measuring changes in gene expression at early time points (minutes).
  • Detect alternate start sites and unannotated genes.
  • Identify genes that are poised for transcriptional activation.
  • Perform in parallel with transcription factor-targeted ChIP to correlate TF binding with changes in transcription.
Transcription Path

TranscriptionPath data reveals four states of RNAPII occupancy.
TranscriptionPath-Seq was performed using chromatin from mouse neuroblastoma cells. Four patterns of RNAPII occupancy were detected; examples are shown above. (A) Promoter Paused and Transcribed -- Transcription is rate-limited post-initiation. (B) Not Paused and Transcribed -- Transcription is rate-limted by RNAAPII recruitment. (C) Promoter Paused and Not Transcibed - These genes are poised for activation, but are not transcribed. In this example, the posed pattern shown is inducible. (D) Not Paused and Not Transcribed - These genese are inactive.

Get additional details and read our TranscriptionPath Application Notes