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Cell-Free DNA (cfDNA) Purification Kit

The Cell-Free DNA (cfDNA) Purification Kit is designed to isolate cell-free DNA
from human plasma and serum samples. Cell-free DNA can serve as a valuable source
of information for non-invasive detection and monitoring of disease states, however,
it is often found in limited amounts in plasma and serum samples. Using Active Motif's
fast, magnetic bead-based protocol, it is easy to isolate and concentrate cell-free
DNA from fresh or frozen samples.

cfDNA_tapestation_web.jpg
Cell-Free DNA (cfDNA) Purification Kit

Cell-Free DNA Purification Kit Highlights

  • Works with fresh and frozen samples
  • Process input volumes from 100 µl to 10 ml
  • Fast, magnetic bead-based purification is compatible with automation
  • Low elution volumes to concentrate the cell-free DNA
  • Compatible with Streck Cell-Free DNA BCT® Tubes

The Cell-Free DNA (cfDNA) Purification Kit contains enough reagents to isolate cell-free DNA from up to 100 ml or 250 ml plasma or serum. To learn more about the kit, click on the Documents, Data or Contents tabs below.

 
Name Format Cat No. Price  
Cell-Free DNA (cfDNA) Purification Kit 100 ml 25503 ¥7,612.50 Buy Now
250 ml 25504 ¥13,122.50 Buy Now

Isolate and Concentrate Cell-Free DNA

Active Motif's Cell-Free DNA (cfDNA) Purification Kit was used to isolate cell-free DNA from 5 ml of healthy human plasma. DNA was eluted in the minimum elution volume to concentrate the sample and quantified using the Qubit dsDNA High Sensitivity Assay. Isolated DNA was run on a TapeStation to assess the size of the purified DNA. Results show the majority of the purified sample is cell-free DNA at the expected size of 150-200 bp.

Cell-Free DNA (cfDNA) Purification Kit.
Figure 1: Use of TapeStation to assess DNA size of isolated cell-free DNA.

 


Robust and Reproducible Data

Plasma samples from four different human donors were tested in duplicate to demonstrate the reproducibility of the Cell-Free DNA (cfDNA) Purification Kit. The kit was used to isolate 1 ml plasma in each reaction. Total nanograms DNA recovered (as determined by Qubit) is shown in the table below. Results show reproducibility between replicates and the robustness of the assay to isolate between 1-100 ng total DNA per 1 ml plasma input.

Reproducibility of cfDNA Purification Kit.
Figure 2: Reproducibility of the Cell-Free DNA (cfDNA) Purification Kit.

 


Automation Compatible

Both the Cell-Free DNA (cfDNA) Purification Kit and a competitor magnetic bead-based cfDNA purification Kit (Competitor T) were used to isolate DNA from 600 µl of plasma from a normal human donor in triplicate using the KingFisher Flex Purification System. Results show the total DNA yield (ng) per ml plasma. The Cell-Free DNA (cfDNA) Purification Kit recovered more DNA than the alternative purification method.

cfDNA Purification Kit with Automation.
Figure 3: Automation compatibility with KingFisher Flex Purification System.

Contents & Storage

The Cell-Free DNA (cfDNA) Purification Kit contains sufficient reagents to isolate cell-free DNA from 100 ml or 250 ml human plasma or serum. Easily process input volumes from 100 µl to 10 ml. Both fresh and frozen plasma can be used, however, fresh plasma tends to have higher yields. Plasma from blood collected with Streck Cell-Free DNA BCT® Tubes must go through a Proteinase K treatment (not included) prior to purification to ensure optimal yields.

The Cell-Free DNA (cfDNA) Purification Kit is shipped and can be stored at room temperature. Please confirm receipt of all reagents upon arrival and store items at the appropriate temperatures as indicated below. All reagents are guaranteed stable for 6 months from date of receipt when stored properly. This kit includes the following components:

  • Lysis/Binding Buffer AM2; Store at RT
  • Wash Buffer AM8; Store at RT
  • Elution Buffer AM6; Store at RT
  • Magnetic Bead Solution; Store at RT