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NEDD4 antibody (rAb), 100 µg
| Clone: 230478F1 |
| Aliases: neural precursor cell expressed developmentally down-regulated 4 |
| Catalog No: 91661 | Format: 100 µg | ¥5,670 | Add to Cart |
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Applications
Application Notes
Applications Validated by Active Motif:
WB: 1:1000-1:5000 dilution
Immunogen
This antibody was raised against the NEDD4 fusion protein.
Buffer
Purified IgG in PBS, pH 7.3, with 50% glycerol and 0.02% sodium azide. Sodium azide is highly toxic.
NEDD4 antibody (rAb) tested by Western blot.
Detection of NEDD4 by Western blot analysis 30 µg of C6 or NIH/3T3 lysates and probed with NEDD4 antibody at 1:5000 dilution.
Background
NEDD4 and similar proteins discovered subsequently became a family of HECT ligases, comprising 9 human proteins including NEDD4, NEDD4-2 (NEDD4L), ITCH, SMURF1, SMURF2, WWP1, WWP2, NEDL1 and NEDL2. NEDD4 is a highly evolutionarily conserved protein from yeast to man and was initially cloned as a highly expressed gene in the early embryonic brain. NEDD4 is frequently overexpressed in many different types of cancer, and decreased levels of NEDD4 can also be associated with cancer. It can be a potential therapeutic target for the treatment of human cancer.
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- TIP-ChIP = Tagmented, Indexed, and Pooled Chromatin Immunoprecipitation;
- WB = Western Blotting
