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Transcriptional Regulation and Cancer

Genetic and epigenetic modifications are fundamental to normal development and alterations in the regulation of gene expression have been reported in a wide range of diseases. The significance of epigenetic alterations in cancer is supported by the fact that they have been found in every tumor type studied to date, both benign and malignant. During tumor initiation and progression, gene regulation becomes altered by the aberrant genetic inactivation/activation of transcription factors and by changes to the epigenome, including a genome-wide loss of DNA methylation, frequent increases in promoter methylation of CpG islands, changes in nucleosome occupancy, and shifts in modification profiles.

Quantitate Transcription Factor activation
Transam Understanding and quantifying transcription factors is essential for the study of cell function in relation to differentiation, brain activity, immune response, inflammation and various disease states such as cancer. The TransAM® Kits are sensitive, non-radioactive transcription factor plate based assays that facilitate the study of transcription factor activation in mammalian tissue and cell extracts. Active Motif offers TransAM® assays for over 40 different targets. More >

Measure transcriptional regulation of any gene
antibodies Choose from over 18,000 promoter reporter constructs from the human genome, allowing you to determine the functional response of the selected promoters to stimuli in nearly any cell-based system. The Go Clone collection uses a novel luciferase reporter making it easy for you to measure the transcriptional and post-transcription regulation of any gene in the genome. More >

Analyze pathway specific transcriptional regulation
chipseq The LightSwitch™ Validated Pathway Collection contains promoter reporter constructs selected from the 18,000-member LightSwitch Promoter Reporter GoClone® Collection. Each collection was created using pathway-specific induction experiments, so all members have been experimentally validated to be well suited to study a specific pathway of interest, such as DNA damage/Apoptosis, inflammation, hypoxia, etc. Validated pathways allow you to screen for promoter activation or develop activity profiles across various signaling pathways for many compounds or conditions in parallel. More >

Study the impact of miRNA’s on gene regulation
miRNA Choose from over 12,000 endogenous human 3´UTR clones that make up the LightSwitch 3´UTR Reporter Collection. These are ideal for performing miRNA target validation, as well as assessing the functional impact of miRNA-3´UTR interactions. In combination with the miRNA Mimics and Inhibitors, which are co-transfected with the 3´UTR Reporter constructs, you have everything needed to validate miRNA targets, measure RNA stability, translation efficiency, and the functional impact of miRNAs on a gene-by-gene basis. More >