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Epigenetics Podcast from Active Motif

Active Motif's Epigenetics Podcast is a lively discussion about the latest tips and techniques for epigenetics research. Podcast host Dr. Stefan Dillinger chats with experts from different fields within epigenetics about the latest research findings, the hottest epigenetics applications, and how to overcome the many challenges of ChIP and other epigenetic assays.









& Disease

Hodaka Fujii headshot

Development of Site-Specific ChIP Technologies (Hodaka Fujii)

Episode 32

In this episode of the Epigenetics Podcast, we caught up with Dr. Hodaka Fujii, Professor of Biochemistry and Genome Biology at Hirosaki University Graduate School of Medicine and School of Medicine, to talk about his work on the development of locus-specific ChIP technologies. We also discuss the story behind how Dr. Fujii got into the field of epigenetics, how he developed iChIP, and how the method was improved over the years. Furthermore, we discuss the development of enChIP and how this method can be used as an alternative to Hi-C.

Christine Cucinotta & Melvin Noe Gonzalez headshots

How the "Fragile Nucleosome" Science Community Came to Life (Christine Cucinotta, Melvin Noe Gonzalez)

Episode 30

In this episode of the Epigenetics Podcast, we caught up with Dr. Christine Cucinotta and Dr. Melvin Noe Gonzalez, members of the organizing committee for the independent "Fragile Nucleosome" scientific community, to talk about how they brought the #fragilenucleosome seminar series and Discord channel to life. In this interview, Christine and Melvin share the story of how the Fragile Nucleosome community got started, what has happened so far, and what the future plans are for the #fragilenucleosome.

Isabelle Mansuy headshot

Epigenetic Influence on Memory Formation and Inheritance (Isabelle Mansuy)

Episode 29

In this episode of the Epigenetics Podcast, we caught up with Professor Isabelle Mansuy, Ph.D. from the University of Zürich and the ETH Zürich, to talk about her work on epigenetic influences on memory formation and inheritance. Check out this interview to learn more about the challenges and obstacles that needed to be overcome to create a novel experimental approach to tackle the questions of whether and which epigenetic factors might influence transgenerational epigenetic inheritance.

Chuan He headshot

Influence of Dynamic RNA Methylation on Gene Expression (Chuan He)

Episode 28

In this episode of the Epigenetics Podcast, we caught up with Dr. Chuan He, John T. Wilson Distinguished Service Professor at the University of Chicago, to talk about his work on the influence of dynamic RNA methylation on gene expression. In this interview, we discuss the story of how the He lab discovered the members of the family of proteins that read, write, and erase RNA modifications and the mechanisms of how those RNA modifications act in the field of epigenetics.

Michelle Trenkmann headshot

How to Publish in Nature: Lessons from the ENCODE Consortium (Michelle Trenkmann, Senior Editor at Nature)

Episode 27

In this episode of the Epigenetics Podcast, we caught up with Dr. Michelle Trenkmann, Senior Editor at Nature. We discussed her work as an editor at Nature and how she contributed to the ENCODE 3 publications, which are the results of the third phase of the ENCODE project. Dr. Trenkmann also talked about how to get your research published in Nature and what it’s like to review high profile scientific articles.

Bill Earnshaw headshot

The Role of Non-Histone Proteins in Chromosome Structure and Function During Mitosis (Bill Earnshaw)

Episode 26

In this episode of the Epigenetics Podcast, we caught up with Professor Bill Earnshaw, Wellcome Trust Principal Research Fellow at the University of Edinburgh, to talk about his work on the role of non-histone proteins in chromosome structure and function during mitosis.

In this interview, we discuss the story on how centromeric proteins were first identified using sera from human scleroderma patients, how the chromosomal passenger complex was discovered, how condensin I and II work together in chromatin loop formation, and much more!

Leonid Mirny headshot

Biophysical Modeling of 3-D Genome Organization (Leonid Mirny)

Episode 23

In this episode of the Epigenetics Podcast, we caught up with Leonid Mirny, Ph.D., from Massachusetts Institute of Technology to talk about his work on biophysical modeling of the 3-D structure of chromatin.

In this interview, we chatted with Dr. Mirny about the details of Hi-C, the development of Micro-C and how it compares to Hi-C, and how biophysical modeling helps to unravel the mechanisms behind loop extrusion.

Bing Ren headshot

Identification of Functional Elements in the Genome (Bing Ren)

Episode 21

In this episode of the Epigenetics Podcast, we caught up with Bing Ren, Ph.D., from the University of California, San Diego and the Ludwig Institute for Cancer Research to talk about his work on identifying functional elements in the genome and higher order genome structure.

Dr. Ren’s lab invented an approach for finding cis-elements that involves the identification of transcription factor binding sites and chromatin modification status genome-wide using chromatin immunoprecipitation-based methods. His group demonstrated that this is an effective approach for genome-wide mapping of cis-elements, and their approach has now been widely adopted in the field. Among many other distinctions, Bing Ren's group was also a major contributor to the ENCODE Project.

In this interview, we discuss the path Bing Ren has taken so far on scientific career, his role in the ENCODE Project and Roadmap Epigenome Consortia, and the discovery of topologically associating domains (TADs).

Tom Moss headshot

Chromatin Structure and Dynamics at Ribosomal RNA Genes (Tom Moss)

Episode 19

In this episode of the Epigenetics Podcast, we caught up with Professor Tom Moss from Université Laval in Québec City, Canada to talk about his work on the chromatin structure and dynamics at ribosomal RNA genes.

Dr. Tom Moss has been a member of the Department of Molecular Biology, Medical Biochemistry, and Pathology at the Laval University School of Medicine since he was recruited from the University of Portsmouth in the United Kingdom in 1986. Since then he focused on the ribosomal transcription factor Upstream Binding Factor (UBF) and how it regulates the chromatin structure at ribosomal RNA genes (rDNA).

UBF binds to the rDNA as a dimer where it leads to six in-phase bends and induces the formation of the ribosomal enhanceosome. This enhanceosome is required for the initial step in formation of an RNA polymerase I initiation complex, and therefore plays an important role in regulating the expression of ribosomal RNA genes.

In this interview, we discuss the function of UBF on the rDNA, how UBF impacts the chromatin landscape at rRNA genes, the role of DNA methylation in this process, and how UBF influences the structure of the nucleolus.

Andrew Pospisilik headshot

Epigenetic Origins Of Heterogeneity And Disease (Andrew Pospisilik)

Episode 18

In this episode of the Epigenetics Podcast, we caught up with Dr. Andrew Pospisilik from the Van Andel Institute in Grand Rapids, Michigan to talk about his work on the epigenetic origins of heterogeneity and disease.

Dr. Andrew Pospisilik worked at the Max Planck Institute for Epigenetics in Freiburg for 8 years and in 2018 he joined the Van Andel Institute as the director of its Center for Epigenetics. At the Van Andel Institute his research focuses on diabetes, neurodegenerative diseases, cancer, and obesity. The goal of the Pospisilik Laboratory is to better understand epigenetic mechanisms of these diseases and the roles of epigenetics in disease susceptibility and heterogeneity.

These areas of medicine are among the most important public health challenges, with the latest estimates suggesting that they impact more than 1 billion people worldwide. Although these diverse conditions are all very different, they are now thought to be caused, at least partially, from alterations in the epigenetic mechanisms that regulate gene expression and metabolism.

This podcast interview covers recent work from the Pospisilik lab on the epigenetics of these complex diseases.

Karol Bomsztyk and Tom Matula headshots

PIXUL: On the Leading Edge of Chromatin Shearing (Karol Bomsztyk and Tom Matula)

Episode 17

In this episode of the Epigenetics Podcast, we caught up with Karol Bomsztyk, M.D. and Tom Matula, Ph.D. from the University of Washington and Matchstick Technologies to talk about their work on DNA and chromatin sonication.

During his career, Karol's research has focused on improving ChIP protocols to make them faster, easier, and higher throughput. First, to make ChIP assays faster, Karol and his lab developed Fast ChIP. More recently, he adjusted this protocol to improve throughput and Matrix ChIP was born. Tom is an expert in the field of ultrasound physics and cavitation and the Director of the Center for Industrial and Medical Ultrasound at the University of Washington.

To further improve and speed up the 96-well Matrix ChIP protocol, Karol and Tom teamed up and co-founded Matchstick Technologies to develop a sonication device that would be able to processes each and every well of a 96-well microplate consistently and quickly. The result of this cooperation is the PIXUL Multi-Sample Sonicator that is now available for order from Active Motif.

PIXUL is an ultrasound-based sample preparation platform that was designed completely from the ground up to provide researchers with an easy-to-use tool that is simple to set up, simple to use, and generates consistent results day in and day out. No other sample preparation platform out there can match the power and convenience of PIXUL.

PIXUL was conceived by an epigenetics researcher and designed and built by ultrasound engineers to take the guesswork out of sample preparation. With PIXUL, sample preparation is no longer an art form, but instead a simple and predictable part of experiments that works every single time.

This interview goes into the mechanism behind sonication-based shearing of DNA and chromatin and highlights how PIXUL is different from existing sonication instruments.

Marcus Buschbeck headshot

Influence of Histone Variants on Chromatin Structure and Metabolism (Marcus Buschbeck)

Episode 16

In this episode of the Epigenetics Podcast, we sat down with Marcus Buschbeck, Group Leader at the Josep Carreras Leukaemia Research Institute in Barcelona, to talk about his work on the histone variant macroH2A, its role in metabolism, and how it contributes to the regulation of chromatin structure.

Histone variants equip chromatin with unique properties and show a specific genomic distribution. The histone variant macroH2A is unique in having a tripartite structure consisting of a N-terminal histone-fold, an intrinsically unstructured linker domain, and a C-terminal macro domain. Recent discoveries show that macroH2A proteins have a major role in nuclear organization, which has the potential to explain how these proteins can act as tumor suppressors, promoters of differentiation, and barriers to somatic cell reprogramming.

We discuss these topics, the mission of the Josep Carreras Leukaemia Research Institute, and much more in this episode.

Lucy Stead headshot

Epigenetics & Glioblastoma: New Approaches to Treat Brain Cancer (Lucy Stead)

Episode 14

In this episode of the Epigenetics Podcast our guest Dr. Lucy Stead, Head of Glioma Genomics at the University of Leeds, discusses her recent work on intratumor heterogeneity in glioblastoma brain tumors. Her research involves a true multidisciplinary approach, including computational genomics, in silico modeling, and functional genomics. She uses this experimental strategy to test whether treatment-resistant cancer cells emerge in recurrent tumors and characterizes them in clinically relevant ways in multiple patients.

And this is just a glimpse of the conversation. Listen to the podcast episode to learn more about this fascinating topic and potential new ways to treat brain cancer.

Ana Pombo headshot

The Interchromatin Network Model (Ana Pombo)

Episode 12

There are many levels of control that contribute to regulating the levels of gene expression. One major mechanism of gene regulation involves organization of the genome within the nucleus. In this episode of the Epigenetics Podcast, our guest Professor Ana Pombo from the Max-Delbrück-Center in Berlin provides insight into her work on the interplay between gene regulation and genome architecture.

Dr. Pombo and her team use different state-of-the-art methods in their research, including cryo-sectioning, to unravel this regulatory network. In 2006, they proposed the "Interchromatin Network Model" of chromosome organization which postulates that chromosome folding is driven by contacts between different genomic regions and chromatin and nuclear landmarks, such as the nuclear lamina. They also used polymer physics modeling to study those mechanisms, which lead to the development of the "Strings and Binders Switch" model of chromatin organization.

Listen to the podcast episode to learn more about this fascinating topic.

Asifa Akhtar headshot

Dosage Compensation in Drosophila (Asifa Akhtar)

Episode 11

For many organisms, from fruit flies to humans, sex is determined by the number of X chromosomes. Females often have two copies of the X and males have one, creating an imbalance in the number of X chromosome genes present in each sex. Different organisms have evolved different strategies for how to cope with this imbalance. For example, in humans, one of the two X chromosomes in every female cell is randomly inactivated, leaving both males and females with one active X chromosome each. However, in Drosophila, the balance is achieved by males overexpressing the genes on their single X chromosome two-fold to bring up the levels of gene expression to match expression from the two X chromosomes in females. Essentially the same end result is achieved in both humans and Drosophila, balanced levels of X chromosome transcription in both males and females, but using two extremely different mechanisms.

In this episode, our guest Dr. Asifa Akhtar, Senior Group Leader and Director at the Max Planck Institute of Immunobiology and Epigenetics in Freiburg, Germany, discussed her lab's recent work on dosage compensation in Drosophila melanogaster. Dr. Akhtar talked about how the MSL complex, and the histone acetyltransferase MOF in particular, contributes to the regulation of the dosage compensation process. Furthermore, she also talked about some potential functions of the conserved MSL proteins in humans and how they are similar to and different from their fruit fly counterparts.

Check out our blog post to learn more.

Jean-Sebastien Annicotte

Diabetes and Epigenetics (Jean-Sébastien Annicotte)

Episode 8

Type 2 Diabetes (T2D) is a chronic metabolic disease that is caused by the failure of beta-cells in the pancreas and insulin resistance in peripheral tissue, and is characterized by high glucose levels in the blood. 382 million people suffer from diabetes worldwide, which makes up >8% of the global population.

Due to this high proportion of people affected, it is of high interest to find a cure for this disease and tremendous efforts have been made on deciphering epigenetic regulations that control metabolic tissue function.

For several years, the research team led by Dr. Jean-Sebastien Annicotte have dissected the molecular links between insulin producing cells, insulin target tissues, and T2D/obesity development. Their research has specifically been focused on the role of cell cycle regulators and their transcriptional co-regulators in the control of metabolic homeostasis, T2D and obesity.

In this podcast episode, we caught up with Dr. Jean-Sebastien Annicotte to discuss his views on type 2 diabetes and the roles of epigenetic mechanisms in regulating this disease.

Check out our blog post to learn more.

Edith Heard headshot

Epigenetics and X-Inactivation (Edith Heard)

Episode 7

In this episode of our Epigenetics Podcast, we sat down with Professor Edith Heard, the Director General of the European Molecular Biology Laboratory (EMBL), to talk about the challenges and goals of her new position as DG of the EMBL and her research on X-inactivation and dosage compensation.

Susan Gasser headshot

Chromatin Organization (Susan Gasser)

Episode 6

In this episode of our Epigenetics Podcast, we caught up with Professor Susan Gasser, director of the Friedrich Miescher Institute in Basel, to talk about her research on heterochromatin, its localization in the nucleus and factors that are involved in the anchoring genomic regions at the nuclear periphery.

Henk Stunnenberg headshot

Epigenomics (Henk Stunnenberg)

Episode 5

In this episode of our Epigenetics Podcast, we chatted with Professor Henk Stunnenberg, head of the Department of Molecular Biology at the Radboud University in Nijmegen, to discuss his scientific work which led him to epigenetics research. Topics include his establishment of the BLUEPRINT and Human Cell Atlas consortia, a European collaborative database of at least 100 reference epigenomes of blood cells from healthy donors and their malignant counterparts, and his feelings about his recent knighthood.

Peter Tessarz headshot

Aging and Epigenetics (Peter Tessarz)

Episode 4

In this episode of our Epigenetics Podcast, we were joined by Dr. Peter Tessarz from the Max Planck Institute for Biology of Ageing in Cologne, and discussed the factors that influence the aging process in humans, how epigenetics comes into play, and how Peter's research can lead to a longer and healthier life.

David Jones headshot

Cancer and Epigenetics (David Jones)

Episode 3

In this episode of our Epigenetics Podcast, we visited Dr. David Jones at the DKFZ in Heidelberg to talk about the implications of epigenetics in cancer. We also talked about David's contributions to the field of pediatric brain tumors and the role of large consortia in doing science today and in the future.

Ada and Don Olins headshots

The Nucleosome: From Atoms to Genomes (Ada and Don Olins)

Episode 2

In this episode of our Epigenetics Podcast we sat down with Ada and Don Olins at the EMBO meeting on "The Nucleosome: From Atoms to Genomes" to talk about their outstanding scientific journey together as a married couple and their perspectives on the future of the chromatin field.