Single-Cell Multiome Service Overview
Single-Cell Multiome allows for both transcriptome analysis and genome-wide detection of open chromatin at the single cell level. Understanding both the gene expression profile and the chromatin state at single-cell resolution can help identify how epigenetic changes instruct gene expression in distinct cell populations.
What are the advantages of using Single-Cell Multiome?
Single-Cell Multiome can be used to identify cell subpopulations with different transcriptome and epigenetic profiles within complex samples, eliminating the need for isolation strategies like FACS or magnetic sorting that could alter the biology due to sample manipulation. For example:
- Identifying novel cell that modulate response to drug treatments (i.e. responders vs. resistant cells)
- Identifying subpopulations of cells with variations in gene expression that can provide insight into developmental trajectories (i.e. brain development, T-helper cell development, B-cell differentiation)
Active Motif’s end-to-end Single-Cell Multiome service includes:
- Preparation of nuclei
- Tn5 tagmentation of open chromatin regions
- Nuclei loaded onto 10x Genomics Chromium Controller
- Barcoding of mRNA and in the same nucleus
- Library generation
- Bioinformatic analysis
Single-Cell Multiome Service Data
Single-Cell Multiome Service FAQs
What is Single-Cell Multiome?
Single-Cell Multiome (scRNA+scATAC) measures both gene expression and maps open chromatin from same cell.
How is single-cell sequencing different from bulk sequencing?
Single-Cell Multiome measures gene expression and open chromatin at single cell resolution such that when examining a heterogenous cell population it can identify variations in gene expression between cell subpopulations within a single sample. Bulk RNA-Seq and ATAC-Seq measure the average expression level or chromatin open/closed state for each gene or locus across a large population of input cells.
Is this service specific to human?
Single-Cell Multiome is compatible with both human and mouse samples. Other species are bioinformatically unsupported (Cell Ranger software is limited to human and mouse).
Is Single-Cell Multiome compatible with FFPE samples?
No. This assay is not compatible with FFPE samples.
How many cells are required for Active Motif's Single-Cell Multiome service?
We ask for 250,000 to 2 million cryopreserved cells or 20-50 mg frozen tissue
What is the read depth for Active Motif's Single-Cell Multiome service?
Per 10X Genomics recommendations, we perform at least 30,000 read pairs per cell. We target 5000 cells, achieving a range of 3000 to 7000 cells.
Single-Cell Multiome Service Documents
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