Recombinant PIAS3 protein

Aliases: E3 SUMO protein ligase PIAS3, ZMIZ5
Catalog No: 82092 Format: 50 µg ¥3,760 Add to Cart
Catalog No: 82692 Format: 1 mg ¥25,600 Add to Cart

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Expressed In: E. coli Protein Species: Human

Contents

A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.

Background

PIAS3 (E3 SUMO-protein ligase PIAS3) is a E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway and the steroid hormone signaling pathway. Involved in regulating STAT3 signaling via inhibiting STAT3 DNA-binding and suppressing cell growth. Enhances the SUMOylating of MTA1 and may participate in its paralog-selective SUMOylation. SUMOylates CCAR2 which promotes its interaction with SIRT1. Diminishes the SUMOylation of ZFHX3 by preventing the colocalization of ZFHX3 with SUMO1 in the nucleus.

Application Notes

This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product.  All available data for this product is shown. 

Protein Details

Recombinant PIAS3 protein that includes full length of human PIAS3 protein (accession number NP_006090.2) was expressed in E. coli and contains an N-terminal His tag with a molecular weight of 70.18 kDa. The purity of the protein is ≥ 80% by SDS-PAGE.

 
Recombinant NSUN1 protein

Recombinant PIAS3 protein
12.5% SDS-PAGE Coomassie staining
MW: 70.18 kDa
Purity: >80%

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.