Histone H4K5ac antibody (pAb)

RRID: AB_2793267
Catalog No: 39583 Format: 200 µl ¥5,610 Buy
Catalog No: 39584 Format: 10 µl ¥1,380 Buy

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Antibody Type:
Polyclonal
Isotype:
Serum
Purification:
None
Host:
Rabbit
Molecular Weight:
8 kDa
Reactivity:
Human, Mouse, Wide Range Predicted

Applications

ChIP Validated Western Blot Validated Immunofluorescence Validated Dot Blot Validated Immunocytochemistry Validated

Application Notes

Applications Validated by Active Motif:
ChIP: 5 - 10 µl per ChIP
ICC/IF: 1:500 - 1:1,000 dilution
WB: 1:2,500 - 1:10,000 dilution

Published Applications

The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:

ChIP
WB
IF

View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.

Immunogen

This Histone H4 acetyl Lys5 antibody was raised against a peptide including acetyl-lysine 5 of histone H4.

Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, an IgG version (Catalog No. 39699) of this antibody that was purified by Protein A Chromatography is also available.

View our Guide to Histone Modifications and Biological Function.

 

Histone H4 acetyl Lys5 antibody tested by ChIP analysis.
Chromatin IP performed using the ChIP-IT® Express Kit (Catalog No. 53008) and HeLa Chromatin (1.5 x 10^6 cell equivalents per ChIP) using 10 µg of Histone H4 acetyl Lys5 pAb or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the indicated gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.

Histone H4 acetyl Lys5 antibody tested by ChIP.
Chromatin IP performed using the ChIP-IT® Express Kit (Catalog No. 53008) and 50 µl of Ready-to-ChIP HeLa Chromatin (Catalog No. 53015) per ChIP. Subsequent to the ChIP reaction, DNA was purified from the immunoprecipitated chromatin and a region of the human GAPDH promoter was amplified by PCR.
    Lane 1: PCR input control.
    Lane 2: ChIP using negative control rabbit IgG.
    Lane 3: ChIP using 10 µl of Histone H4 acetyl Lys5 antibody.

Histone H4 acetyl Lys5 antibody tested by immunofluorescence.
Top left: HeLa cells stained with Histone H4 acetyl Lys5 antibody (1:1,000). Top right: Same cells stained with alpha Tubulin mAb (Clone 5-B-1-2). Bottom left: Stained with DAPI. Bottom right: Merge of all 3 images.

Histone H4 acetyl Lys5 antibody tested by Western blot.
HeLa nuclear extract (20 µg per lane) probed with Histone H4 acetyl Lys5 polyclonal antibody (1:5,000).
    Lane 1: No treatment.
    Lane 2: Cells treated with sodium butyrate.

Histone H4 acetyl Lys5 antibody tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H4 acetyl Lys5 antibody for acetyl Lys5 histone H4. Acetylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at a dilution of 1:5,000. The amount of peptide (picomoles) spotted is indicated next to each row.

   Lane 1: acetyl-Lys5 peptide.  Histone H4 acetyl Lys5 antibody tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H4 acetyl Lys5 antibody for acetyl Lys5 histone H4. Acetylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at a dilution of 1:5,000. The amount of peptide (picomoles) spotted is indicated next to each row.

   Lane 1: acetyl-Lys5 peptide.   Lane 2: unmodified Lys5 peptide.
   Lane 3: acetyl-Lys8 peptide.   Lane 4: unmodified Lys8 peptide.
   Lane 5: acetyl-Lys12 peptide.  Lane 6: unmodified Lys12 peptide.
   Lane 7: acetyl-Lys16 peptide.  Lane 8: unmodified Lys16 peptide.Lane 2: unmodified Lys5 peptide.
   Lane 3: acetyl-Lys8 peptide.   Lane 4: unmodified Lys8 peptide.
   Lane 5: acetyl-Lys12 peptide.  Lane 6: unmodified Lys12 peptide.
   Lane 7: acetyl-Lys16 peptide.  Lane 8: unmodified Lys16 peptide.

Background

Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points; it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; they play a major role in regulating gene expression.

Lysine N-ε-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. Histone H4 molecules acetylated at Lys5 or Lys8 are distributed in overlapping, but non-identical, islands throughout the euchromatic chromosome arms.

Positive Control

Active Motif's HeLa acid extract (Sodium butyrate treated) (Catalog No. 36202) can be used as a positive control for Histone H4 acetyl Lys5 antibody.

qPCR Primers

Active Motif's Human Positive Control Primer Set ACTB-2 (Cat. No. 71005) and Human Negative Control Primer Set 1 (Cat. No. 71001) have been validated for performing qPCR and endpoint PCR when this antibody has been used for ChIP on human samples. For ChIP experiments with this antibody on mouse samples, the Mouse Positive Control Primer Set Actb-2 (Cat. No. 71017) and Mouse Negative Control Primer Set 1 (Cat. No. 71011) have been validated for both qPCR and endpoint PCR.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H4K5ac antibody (pAb).

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting