ATM phospho Ser1981 antibody (mAb)

RRID: AB_2793244
Clone: 10H11.E.12
Aliases: Ataxia Telangiectasia Mutated, AT1, ATA, ATC, ATD, ATE, ATDC, TEL1
Catalog No: 39529 Format: 200 µl ¥5,500 Buy
Catalog No: 39530 Format: 10 µl ¥1,380 Buy

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Antibody Type:
Monoclonal
Isotype:
IgG1, k
Purification:
Ascites
Host:
Mouse
Molecular Weight:
370 kDa
Reactivity:
Human, Mouse

Applications

Western Blot Validated Immunofluorescence Validated Immunocytochemistry Validated

Application Notes

Applications Validated by Active Motif:
ICC/IF: 1:500 dilution

This antibody is also available as an AbFlex® engineered recombinant antibody. For details on the corresponding AbFlex Recombinant Antibody, see Catalog No. 91207.

Published Applications

The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:

IF

View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.

Immunogen

This ATM phospho Ser1981 antibody was raised against a peptide containing phospho-serine 1981 of human ATM.

Buffer

Ascites containing 30% glycerol and 0.035% sodium azide.

 
ATM phospho Ser1981 antibody (mAb) tested by immunofluorescence.

ATM phospho Ser1981 antibody (Clone 10H11.E.12) tested by immunofluorescence.
HeLa cells stained with ATM phospho Ser1981 antibody (Clone 10H11.E.12) at a 1:500 dilution using using MAX Stain™ Immunofluorescence Tools.
Panel A: Normal HeLa cells.
Panel B: HeLa cells treated with 3 Gy ionizing radiation and collected 30 minutes post-exposure.
Top images: Cells stained with DAPI.
Middle images: Same cells stained with ATM phospho Ser1981 mAb (Clone 10H11.E.12).
Bottom images: Merge of the two images above.
Note the Panel B-middle image, which shows intense nuclear clustering of ionizing radiation-induced phosphorylation of ATM at serine 1981. In contrast, Panel A-middle image shows no detectable phosphorylated ATM.

Background

ATM – Ataxia Telangiectasia Mutated is a PI3-family protein kinase and a critical cell cycle checkpoint protein. ATM and a related protein, ATR, play crucial roles in the maintenance of genome integrity and the response to DNA damage. Upon activation, ATM phosphorylates itself at serine 1981 and then phosphorylates a number of downstream proteins (e.g. BRCA1, CHK2, H2AX, p53, NBS1 and SMC1) resulting in cell cycle arrest and the initiation of DNA damage repair. Loss of function of ATM is causal to a variety of syndromes involving increased incidence of several cancers.

Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for ATM phospho Ser1981 antibody (Clone 10H11.E.12).

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting