ChIP-Seq & ChIP-chip验证抗体

经过试验验证可以用于ChIP或者ChIP-chip的抗体

Chromatin immunoprecipitation (ChIP) is widely used to detect protein/DNA interactions. Because only a small percentage of antibodies work well in ChIP, identifying a good antibody is often the biggest challenge in getting the technique to work. Most commercially available “ChIP Validated” antibodies are validated using qPCR to show enrichment of specific genomic locations. Today, more and more investigators are performing ChIP on a genome-wide scale using ChIP-Seq or ChIP-chip. The antibody requirements needed to get good results on a genome-wide scale can be more stringent. Lower background and better enrichments translate into cleaner, more interpretable genome-wide data.

Active Motif now has a ChIP-Seq and ChIP-chip antibody validation program so that customers who are interested in these techniques can feel confident that our antibodies will perform well in these techniques. In addition, our Epigenetic Services team offers ChIP Antibody Validation Services in which we will determine if your antibody is suitable for use in ChIP, ChIP-Seq or ChIP-chip.

Our ChIP-Seq/ChIP-chip validated antibodies have been tested in either, and often both, applications. As we have found that 100% of the antibodies that have worked in ChIP-chip have also performed well in ChIP-Seq, we strongly recommend all antibodies that have been tested only in ChIP-chip for ChIP-Seq applications. The validated antibodies are listed in the table below. Click on the antibody name to see its complete information, including images selected from the genome-wide data sets.

 
Name Isotype Reactivity Appl. Format Cat No. Price  
Androgen Receptor antibody (pAb) IgG Human, Mouse ChIP, ChIP-Seq, WB 100 µl 39781 ¥4,895 Buy
10 µl 39782 ¥1,155 Buy
ASH2L antibody (pAb) Serum Human, Wide Range Predicted ChIP, ChIP-Seq, WB 100 µl 39099 ¥4,895 Buy
10 µl 39100 ¥1,155 Buy
AbFlex® BMI-1 antibody (rAb) IgG2a Human, Mouse ChIP, ChIP-Seq, WB 100 µg 91195 ¥4,235 Buy
10 µg 91196 ¥880 Buy
BMI-1 antibody (mAb) IgG2a Human, Mouse ChIP, ChIP-Seq, IF, IHC, WB 100 µg 39993 ¥4,785 Buy
10 µg 39994 ¥1,155 Buy
Boris / CTCFL antibody (pAb) IgG Human ChIP, ChIP-Seq, WB 100 µl 39851 ¥4,895 Buy
10 µl 39852 ¥1,155 Buy
BRD2 antibody (pAb) IgG Human ChIP, ChIP-Seq 100 µl 61797 ¥4,895 Buy
10 µl 61798 ¥1,155 Buy
BRD3 antibody (pAb) IgG Human, Mouse ChIP, ChIP-Seq, WB 100 µl 61489 ¥4,895 Buy
10 µl 61490 ¥1,155 Buy
AbFlex® BRD4 antibody (rAb) IgG Human ChIP, ChIP-Seq 100 µg 91301 ¥4,235 Buy
10 µg 91302 ¥880 Buy
BRD4 antibody (pAb) IgG Human, Mouse ChIP, ChIP-Seq, IHC, WB 100 µl 39909 ¥4,895 Buy
50 µl 39010 ¥2,860 Buy
10 µl 39910 ¥1,155 Buy
BRD8 / SMAP2 antibody (pAb) IgG Human ChIP, ChIP-Seq, WB 100 µl 61007 ¥4,895 Buy
10 µl 61008 ¥1,155 Buy