ASH2L antibody (pAb)

RRID: AB_2615057
Aliases: ASH2, Bre2, ASH2L1, ASH2L2
Catalog No: 39099 Format: 100 µl ¥5,610 Buy
Catalog No: 39100 Format: 10 µl ¥1,380 Buy

Request a quote for a bulk order Request Quote

Product Review Submit a Review

Antibody Type:
Polyclonal
Isotype:
Serum
Purification:
None
Host:
Rabbit
Molecular Weight:
77 kDa
Reactivity:
Human, Wide Range Predicted

Applications

ChIP Validated ChIP-Seq Validated Western Blot Validated

Application Notes

Applications Validated by Active Motif:
ChIP: 10 µl per ChIP
ChIP-Seq: 10 µl each
WB*: 1:500 - 1:2,000 dilution

ChIP-Seq validation was performed by Active Motif's Epigenetics Services; the complete data set is available in the UCSC Genome Browser by clicking here.

*Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.

Published Applications

The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:

ChIP
WB

View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.

Immunogen

This ASH2L antibody was raised against full-length recombinant human ASH2L protein.

Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

 
ASH2L antibody (pAb) tested by ChIP-Seq.

ASH2L antibody (pAb) tested by ChIP-Seq.
ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 30 ug of chromatin from undifferentiated hESC cells and 10 µl of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 20 million sequence tags were mapped to identify ASH2L binding sites. The image shows binding across a region of chromosome 12. You can view the complete data set in the UCSC Genome Browser, starting at this specific location, here.

ASH2L antibody (pAb) tested by Western blot.

ASH2L pAb tested by Western blot.
ASH2L detection by Western blot. The analysis of ASH2L was performed using HeLa nuclear extract and ASH2L pAb at a 1:2,000 dilution.

Background

ASH2L (absent, small or homeotic discs 2) is a trithorax protein and is a component of different protein complexes, including i.e. mixed-lineage leukemia (MLL) oncoproteins, which contain histone methyltransferase activity. These complexes trimethylate the lysine 4 of histone H3. Histone H3 trimethyl Lys4 is one of histone modifications that are part of elaborate mechanisms that evolved to control the structure of the chromatin.

Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for ASH2L antibody.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with ASH2L antibody (pAb).

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting