AbFlex® Mili / PiwiL2 antibody (rAb)

RRID: AB_2793820
Catalog No: 91253 Format: 100 µg ¥4,240 Buy
Catalog No: 91254 Format: 10 µg ¥940 Buy

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Antibody Type:
Recombinant
Isotype:
IgG2a
Purification:
Protein A Chromatography
Host:
Mouse
Molecular Weight:
110 kDa
Reactivity:
Mouse

Applications

Western Blot Validated

Application Notes

Applications Validated by Active Motif:
WB: 0.2 - 2 µg/ml

AbFlex® recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental clone, see Catalog No. 61143.

Immunogen

This antibody was raised against full length mouse MILI  / PIWIL2 (Miwi like /  piwi-like 2) recombinant protein.

Buffer

Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035% sodium azide, 30% glycerol.

 
AbFlex<sup>®</sup> Mili / PiwiL2 antibody (rAb) tested by Western blot.

AbFlex® Mili / PiwiL2 antibody (rAb) tested by Western blot.
20 µg of mouse testis tissue extract was run on SDS-PAGE and probed with AbFlex Mili / PiwiL2 antibody at 2 µg/ml.

Background

AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis Tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex® Mili / PiwiL2 antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells.

Mili / PiwiL2 is a member of the PIWI/AGO subfamily of RNA-binding proteins. Mili / PiwiL2 contains a Piwi and an AGO domain and binds a class of RNA called piwi-interacting RNA (piRNA), which are 24 to 30 nucleotides in length and are derived primarily derived from transposons and other repeated sequence elements. Mili / PiwiL2 is required for methylation and inactivation of retrotransposons. Mice deficient in Mili / PiwiL2 are sterile, as it is also required for spermatogenesis. It also positively regulates translation, and is required for germ line stem cell division and differentiation.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting