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AbFlex® Histone H3K27me3 antibody (rAb)
| RRID: AB_2793791 |
| Catalog No: 91167 | Format: 100 µg | ¥4,400 | Add to Cart |
| Catalog No: 91168 | Format: 10 µg | ¥990 | Add to Cart |
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Applications
Published Applications
The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:
ChIP-qPCR
View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.
Immunogen
This Histone H3 trimethyl Lys27 antibody was raised against a peptide containing trimethyl Lys27 of human Histone H3.
Buffer
140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035% sodium azide, and 30% glycerol. Sodium azide is highly toxic.
AbFlex® Histone H3K27me3 antibody (rAb) tested by Western blot.
20 µg of HeLa cell nuclear extract* was run on SDS-PAGE and probed with AbFlex Histone H3K27me3 antibody at 0.5 µg/ml.
AbFlex® Histone H3K27me3 antibody specificity is shown by Dot Blot.
Recombinant proteins were spotted onto PVDF as follows: Lane 1 - Histone H3 (C110A), Cat. No. 31207; Lane 2 - Histone H3K27me1 (MLA), Cat. No. 31621; Lane 3 - Histone H3K27me2 (MLA), Cat. No. 31597; Lane 4 - Histone H3K27me3 (MLA), Cat. No. 31579. Lane 5 - Histone H3K4me1 (MLA), Cat. No. 31619; Lane 6 - Histone H3K4me2 (MLA), Cat. No. 31595; Lane 7- Histone H3K4me3 (MLA), Cat. No. 31600; Lane 8 - Histone H3K9me1 (EPL), Cat. No. 31281; Lane 9 - Histone H3K9me2 (EPL), Cat. No. 31596; Lane 10 - Histone H3K9me3 (EPL), Cat. No. 31601; Top Panel was probed with Histone H3 antibody (mAb) at 2 µg/ml. Bottom Panel was probed with AbFlex Histone H3K27me3 antibody at 2 µg/ml.
AbFlex® Histone H3K27me3 antibody (rAb) tested by CUT&Tag
CUT&Tag was performed using 100,000 K562 cells and sequenced using 38 base-pair, paired-end reads on the Illumina NovaSeq. Data was collected from 3 million reads, and H3K27me3 data is shown for Chromosome 21.
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- TIP-ChIP = Tagmented, Indexed, and Pooled Chromatin Immunoprecipitation;
- WB = Western Blotting
