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Recombinant CUL5 protein
| Aliases: VACM-1, Vasopressin Activated Calcium Mobilizing Receptor 1, Cullin-5, CUL-5, VACM1 |
| Catalog No: 82103 | Format: 50 µg | ¥3,760 | Add to Cart |
| Catalog No: 82703 | Format: 1 mg | ¥23,600 | Add to Cart |
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Expressed In: E. coli Protein Species: Human
Contents
A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.
Background
CUL5 (Cullin-5) is a core component of multiple cullin-5-RING E3 ubiquitin-protein ligase complexes (ECS complexes, also named CRL5 complexes), which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. Acts a scaffold protein that contributes to catalysis through positioning of the substrate and the ubiquitin conjugating enzyme. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable SOCS box-containing substrate recognition component. As part of some ECS complex, promotes ‘Lys-11’-linked ubiquitination and degradation of BTRC. As part of a Mult subunit ECS complex, polyubiquitinates monoubiquitinated POLR2A.
Application Notes
This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.
Protein Details
Recombinant CUL5 protein that includes full length of human CUL5 protein (accession number NP_003469.2) was expressed in E. coli and contains an N-terminal GST tag with a molecular weight of 117.78 kDa. The purity of the protein is ≥ 80% by SDS-PAGE.
Recombinant CUL5 protein
10% SDS-PAGE Coomassie staining
MW: 117.78 kDa
Purity: ≥ 80%
Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee
This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.
