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Recombinant KRAS (1-186) protein
| Aliases: GTPase KRas, KRAS1, KRAS2, Kirsten Rat Sarsoma Viral Oncogene Homolog, C-Ki-Ras, KRAS3B, K-Ras P21 Protein |
| Catalog No: 81475 | Format: 50 µg | ¥3,600 | Buy |
| Catalog No: 81575 | Format: 1 mg | ¥22,680 | Buy |
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Expressed In: E. coli Protein Species: Human
Contents
A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.
Background
KRAS (GTPase KRas) protein is a GDP/GTP binding protein, and the more important genes of the same family include HRAS and NRAS. KRAS is activated when combined with GTP and closed when combined with GDP. KRAS can be transiently activated by growth factors or tyrosine kinases (such as EGFR). The activated KRAS can activate downstream signals such as PI3K-AKT-mTOR that control cell production pathway, and the RAS-RAF-MEK-ERK signaling pathway that controls cell proliferation. Mutant KRAS will continue to be activated even without activation of kinases such as EGFR, resulting in continued cell proliferation and eventually canceration. KRAS mutations are found in a variety of tumors, the most common ones include lung cancer and pancreatic cancer.
Application Notes
This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.
Protein Details
Recombinant KRAS (1-186) protein that includes amino acids 1-186 of human KRAS protein (accession number NP_203524.1 was expressed in E. coli cells with an C-terminal 6xHis tag. The molecular weight of the protein is 22.36 kDa.
Recombinant KRAS (1-186) protein gel.
12.5% SDS-PAGE gel stained with Coomassie Blue
MW: 22.36 kDa
Purity: >90%
GTPase-Glo assay for KRAS (1-186) activity
1 μM rGTP and 0.5 mM DTT was incubated with different concentrations of KRAS (1-186) in GTPase/ GAP Buffer for 60 minutes at room temperature, the total reaction volume was 5µl. To the completed GTPase reactions, we added 5µl of reconstituted GTPase-Glo™ Reagent to each well and incubated the plate for 30 minutes. Then detection Reagent (10µl) was added, reactions were incubated for 10 minutes and luminescence was measured.
Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee
This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.
