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Recombinant RPS6KA2/RSK3 protein
| Aliases: ribosomal S6 kinase |
| Catalog No: 81408 | Format: 20 µg | ¥3,840 | Add to Cart |
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Expressed In: Baculovirus Protein Species: Human
Contents
A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.
Background
RPS6KA2 is a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains two non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. RPS6KA2 acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of transcription factors, regulates translation, and mediates cellular proliferation, survival, and differentiation. Diseases associated with RPS6KA2 include Coffin-Lowry Syndrome and Dyskeratosis Congenita, Autosomal Dominant 3.
Application Notes
This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.
Protein Details
Recombinant RPS6KA2 / RSK3 protein was expressed in baculovirus expression system as the full-length protein (accession number NP_066958.2) with a N-terminal FLAG tag. The molecular weight of the protein is 84.5 kDa.
References
Human
Recombinant RPS6KA2 / RSK3 protein
10% SDS-PAGE Coomassie staining
MW: 84.5 kDa
Purity: ≥ 90%
HTRF assay for RPS6KA2 / RSK3 protein activity
1 µM STK S1 substrate was incubated with different concentrations of RPS6KA2 / RSK3 protein in a 10 µl reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, and 100 µM ATP for 1 hour. The 10 µl detection reagents containing anti-STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.
HTRF assay for RPS6KA2 / RSK3 protein activity
1 µM STK S1 substrate was incubated with different concentrations of RPS6KA2 / RSK3 protein in a 10 µl reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, and 100 µM ATP for 1 hour. The 10 µl detection reagents containing anti-STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.
Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee
This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.
