Recombinant SARS-CoV-2 NSP8 protein

Catalog No: 81316 Format: 50 µg ¥3,600 Buy
Catalog No: 81616 Format: 1 mg ¥24,000 Buy

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Expressed In: E. coli Protein Species: SARS-CoV-2

Contents

A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.

Background

Coronavirus replication and transcription machinery involves multiple virus-encoded nonstructural proteins (nsp). Coronaviruses are enveloped positive-stranded RNA viruses with the largest currently known RNA genomes. Expression of their genomes begins with the translation of two large replicase polyproteins, pp1a (>4,000 residues) and pp1ab (>7,000 residues), which are encoded by the viral replicase gene that comprises open reading frame 1a (orf1a) and orf1b1. pp1a and pp1ab are extensively processed by orf1a-encoded proteases to yield 15 or 16 mature nonstructural (replicase) proteins that assemble to form the membrane-associated viral replication and transcription machinery, which is vital to the viral life cycle. SARS-CoV-2 NSP8 (Nonstructural Protein 8) is one of the nonstructural proteins encoded by SARS-CoV-2 orf1ab. According to researches, the RNA-dependent RNA polymerase (RdRp, also known as NSP12), catalyzes the synthesis of viral RNA and thus plays a central role in the replication and transcription cycle of COVID-19 virus, possibly with the assistance of NSP7 and NSP8 as co-factors.

Application Notes

Recombinant SARS-CoV-2 NSP8 protein is suitable for use in the study of SARS-CoV-2. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product.  All available data for this product is shown.

Protein Details

Recombinant SARS-CoV-2 NSP8 protein was expressed in E.coli cells as the full length protein (accession number YP_009725304.1) with a C-terminal Avi and a C-terminal 6×His tag. The predicted molecular weight of the protein is 21.89 kDa.

 
Recombinant SARS-CoV-2 NSP8 protein gel

Recombinant SARS-CoV-2 NSP8 protein gel
12.5% SDS-PAGE with Coomassie blue staining

MW: 21.89 kDa

Purity: >90%

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is guaranteed for 6 months from date of arrival.