Recombinant ALKBH1 protein, FLAG-Tag

Aliases: AlkB Homolog 1, Histone H2A Dioxygenase
Catalog No: 81308 Format: 20 µg ¥3,720 Buy
Catalog No: 81608 Format: 1 mg ¥32,000 Buy

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Expressed In: Baculovirus Protein Species: Human

Contents

A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.

Background

ALKBH1 (AlkB Homolog 2, Alpha-Ketoglutarate Dependent Dioxygenase) is a member of ALKBH protein family. It can repair alkylated DNA and RNA containing 1-methyladenine and 3-methylcytosine by oxidative demethylation. ALKBH1 can also repair alkylated DNA containing 1-ethenoadenine (in vitro) and has strong preference for double-stranded DNA. It can release modified bases from both DNA and RNA. This oxidative demethylation requires molecular oxygen, alpha-ketoglutarate and iron.

Application Notes

This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.

Protein Details

Full length ALKBH1 protein (accession number NP_006011.2) was expressed in a baculovirus system with a C-terminal FLAG-Tag. The molecular weight of this protein is 45.5 kDa.

 
Recombinant ALKBH1 FLAG-Tag protein gel

Recombinant ALKBH1 FLAG-Tag protein gel
10% SDS-PAGE with Coomassieblue staining

MW: 45.5 kDa

Purity: >88%

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is guaranteed for 6 months from date of arrival.