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Recombinant SETD3 protein
| Aliases: SET Domain Containing 3, Actin Histidine Methyltransferase, C14orf154, EC 2.1.1.85 |
| Catalog No: 81279 | Format: 20 µg | ¥3,720 | Buy |
| Catalog No: 81979 | Format: 1 mg | ¥32,000 | Buy |
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Expressed In: Baculovirus Protein Species: Human
Contents
A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.
Background
SETD3 (SET Domain Containing 3, Actin Histidine Methyltransferase), is a protein-histidine N-methyltransferase that specifically mediates methylation of actin at ‘His-73’. Histidine methylation of actin is required for smooth muscle contraction of the laboring uterus during delivery. According to researches, SETD3 does not have proteinlysine N-methyltransferase activity and probably only catalyzes histidine methylation of actin.
Application Notes
Recombinant SETD3 protein is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.
Protein Details
Full length human SETD3 protein (accession number NP_115609.2 was expressed in a baculovirus expression system and contains an N-terminal FLAG-Tag. The molecular weight of SETD3 is 68.5 kDa.
Recombinant SETD3 protein gel
10% SDS-PAGE Coomassie staining
MW: 68.5 kDa
Purity: >90%
MTase-Glo Assay for Recombinant SETD3 protein methyltransferase activity
1 µM beta-actin peptide (66-TLKYPIEHGIVTNWD-80) and 1 µM SAM were incubated with different concentrations of recombinant SETD3 protein in an 8 µl reaction system containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP for 1 hr. 5×MTase-Glo Reagent was added to the products and incubated for 30 min. Then MTase-Glo Detection was added and luminescence were read after another 30 min incubation. SAH standard curve (0-1 µM) was performed following the same protocol.
MTase-Glo Assay for Recombinant SETD3 protein methyltransferase activity
1 µM beta-actin peptide (66-TLKYPIEHGIVTNWD-80) and 1 µM SAM were incubated with different concentrations of recombinant SETD3 protein in an 8 µl reaction system containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP for 1 hr. 5×MTase-Glo Reagent was added to the products and incubated for 30 min. Then MTase-Glo Detection was added and luminescence were read after another 30 min incubation. SAH standard curve (0-1 µM) was performed following the same protocol.
Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee
This product is guaranteed for 6 months from date of arrival.
