Cart
Products
BRD4 antibody (pAb)
| RRID: AB_3216369 |
| Aliases: CAP, MCAP, HUNK1, HUNKI |
| Catalog No: 65713 | Format: 100 µg | ¥5,670 | Add to Cart |
| Catalog No: 65913 | Format: 50 µg | ¥3,250 | Add to Cart |
| Catalog No: 65714 | Format: 10 µg | ¥1,430 | Add to Cart |
Request a quote for a bulk order Request Quote
Product Review Submit a Review
Applications
Application Notes
Applications Validated by Active Motif:
*WB: 0.5 - 2.0 µg/ml dilution
*For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.05% Tween 20 to all blocking solutions may also reduce background. Individual optimization may be required.
This antibody target is also available as an AbFlex® engineered recombinant antibody. Catalog No. 91301.
Immunogen
This BRD4 antibody was raised against a peptide within the C-terminal region of human BRD4.
Buffer
Purified rabbit IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
BRD4 antibody (pAb) tested by Western blot.
Nuclear extract of Hela (40 µg / lane) was probed with BRD4 antibody (pAb) at 0.5 µg/mL.
Background
BRD4 (Bromodomain-containing protein 4, aka HUNK1) is a chromatin-associated protein with two bromo domains that binds acetylated histone H3 and H4. BRD4 is homologous to Xenopus MCAP, and like MCAP, BRD4 associates with chromosomes during mitosis, predominantly in the non-centromeric regions. A chromosomal aberration involving BRD4 (producing a BRD4-NUT fusion protein) is found in a rare, aggressive, and lethal carcinoma arising in midline organs of young people. BRD4 is involved in the expression of extracellular matrix gene expression, and its misregulation may be involved in breast cancer progression.
Positive Control
Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for BRD4 antibody.
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- TIP-ChIP = Tagmented, Indexed, and Pooled Chromatin Immunoprecipitation;
- WB = Western Blotting
