KDM2B antibody (mAb)

RRID: AB_3216371
Clone: K2K842-13
Aliases: FBXL10
Catalog No: 65699 Format: 100 µg ¥5,670 Add to Cart
Catalog No: 65700 Format: 10 µg ¥1,430 Add to Cart
Catalog No: 65999 Format: 50 µg ¥3,250 Add to Cart

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Antibody Type:
Monoclonal
Isotype:
IgG
Purification:
Protein A Chromatography
Host:
Mouse
Molecular Weight:
100 kDa
Reactivity:
Human, Mouse

Applications

Western Blot Validated

Application Notes

Applications Validated by Active Motif:
WB: 0.5 - 2 µg/ml dilution

Immunogen

This KDM2B antibody was raised against a peptide containing human lysine-specific demethylase 2B of isoform a.

Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

 
KDM2B antibody (mAb) tested by Western blot.

KDM2B antibody (mAb) tested by Western blot.
Detection of KDM2B by Western blot analysis.
20 µg of HEK293 nuclear extract was run on SDS-PAGE and probed with 0.5 µg of KDM2B antibody.

Background

KDM2B (lysine (K)-specific demethylase 2B), also known as FBXL10 (F-box and leucine-rich repeat protein 10) is a histone demethylase that preferentially demethylates trimethylated lysine 4 (K4me3) and dimethylated lysine 36 (K36me2) of histone H3. KDM2B displays weak or no activity for mono- and trimethylated H3K36. KDM2B preferentially binds the transcribed region of ribosomal RNA and represses the transcription of ribosomal RNA genes which results in inhibition of cell growth and proliferation. KDM2B may also serve as a substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • TIP-ChIP = Tagmented, Indexed, and Pooled Chromatin Immunoprecipitation;
  • WB = Western Blotting