DNA-RNA Hybrid antibody (mAb)

Application Notes

Applications Validated by Active Motif:
DB: 1 µg/ml.

Published Applications

The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:

ChIP-Seq
DNA-RNA Immunoprecipitation (DRIP)
IP
ICC / IF
EMSA
FISH

NOTE: Clone S9.6 has been published for use in multiple applications, however all of these have not been internally tested by Active Motif. For details see the list of references below. View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.

Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

References

Phillips, D.D., et. al. (2013). “The sub-nanomolar binding of DNA-RNA-hybrids by the single chain Fv fragment of antibody S9.6.”, J. Mol. Recognit. 26(8): 376-381.
Gino, P.A., et. al. (2012). “R-loop formation is a distinctive characteristic of unmethylated human CpG island promotors.” Mol. Cell. 45(6): 814-825.
Bhatia, V., et. al. (2014). “BRCA2 prevents R-loop accumulation and associates with TREX-2 mRNA export factor PCID2.” Nature 511(7509): 362-5.
Koo, C.X., et. al. (2015). “RNA polymerase III regulates cytosolic RNA:DNA hybrids and intracellular microRNA expression.” J. Biol. Chem. 290(12):7463-73.
Tran, H.V., et. al. (2013). “Antibodies directed to RNA/DNA hybrids: an electrochemical immunosensor for microRNAs detection using graphene-composite electrodes.” Anal. Chem. 85(17):8469-74.
Hu, Z., et. al.. (2006). “An antibody-based microarray assay for small RNA detection.” Nucleic Acids Res. 34(7): 52.
Szekvolgyi, L., et. al. (2007). “Ribonucleoprotein-masked nicks at 50-kpb intervals in the eukaryotic genomic DNA.” Proc. Natl. Acad. Sci. (104(38): 14964-9.

Background

DNA-RNA hybrids are a natural occurrence within eukaryotic cells, with levels of these hybrids increasing at sites with high transcriptional activity, such as during transcription initiation, repression, and elongation. Because RNA-DNA hybrids influence genomic instability, the S9.6 antibody is a useful reagent to help study the consequences of R-loops and lesions formed by these hybrids during DNA replication or other cellular processes. In addition, the S9.6 antibody is effective in recognizing RNA-DNA hybridization for microarray studies.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT^® High Sensitivity Kit or our magnetic bead-based ChIP-IT^® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with DNA-RNA Hybrid antibody (mAb).

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

• ChIP = Chromatin Immunoprecipitation;
• ChIP = ChIP Sequencing;
• CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
• CUT&Tag = Cleavage Under Targets and Tagmentation;
• DB = Dot Blot;
• ELISA = Enzyme-linked Immunosorbent Assay;
• EMSA = Electrophoretic Mobility Shift Assay
• FC = Flow Cytometry;
• ICC = Immunocytochemistry;
• IF = Immunofluorescence;
• IHC = Immunohistochemistry;
• IP = Immunoprecipitation;
• MeDIP = Methyl-DNA Immunoprecipitation;
• NEU = Neutralizing;
• WB = Western Blotting