KLF6 antibody (pAb)

RRID: AB_2793565
Aliases: GBF, ZF9, BCD1, CBA1, CPBP, PAC1, ST12, COPEB
Catalog No: 61243 Format: 100 µg ¥5,500 Buy
Catalog No: 61244 Format: 10 µg ¥1,380 Buy

Request a quote for a bulk order Request Quote


Product Review Submit a Review


Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Protein A Chromatography
Host:
Rabbit
Molecular Weight:
40 kDa
Reactivity:
Mouse

Applications

Western Blot Validated

Application Notes

Applications Validated by Active Motif:
WB*: 0.5 - 2 µg/ml dilution

The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

*Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.

Immunogen

This antibody was raised against a peptide within the internal region of human KLF6.

Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide.

 

KLF6 pAb tested by Western blot.
3T3-L1 nuclear extract extract following stimulation with a defined hormonal mixture (DMSO, dexamethasone, insulin, and IBMX) at 30 µg per lane was probed with KLF6 pAb at a dilution of 2 µg/ml .
    Lane 1: Day 0.
    Lane 2: Day 10 - fully differentiated adipocytes.

Background

KLF6 (Kruppel-like factor 6) is member of the Kruppel-like family of transcription factors and is a zinc finger protein that acts as a transcriptional activator and functions as a tumor suppressor. Could play a role in B-cell growth and development. Multiple transcript variants encoding different isoforms have been found for this gene, some of which are implicated in carcinogenesis.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting