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Histone H3K4me3 antibody (pAb)
| RRID: AB_2687512 |
| Catalog No: 39915 | Format: 100 µg | ¥5,830 | Add to Cart |
| Catalog No: 39016 | Format: 50 µg | ¥3,360 | Add to Cart |
| Catalog No: 39916 | Format: 10 µg | ¥1,430 | Add to Cart |
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Applications
Published Applications
The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:
ChIP-qPCR, ChIP-Seq
Native ChIP
ICC/IF
WB
View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.
Immunogen
This Histone H3 trimethyl Lys4 antibody was raised against a peptide including trimethyl-lysine 4 of histone H3.
Buffer
Purified IgG in PBS (pH 7.5) with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, an unpurified serum version (Catalog No. 39159) of this antibody is also available.
Histone H3K4me3 antibody tested by ChIP-Seq
Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with Acute Myelocytic Leukemia (AML) cell line chromatin and 4 µg of Histone H3K4me3 antibody. ChIP DNA was sequenced on the Illumina NextSeq and 14.5 million sequence tags were mapped to identify Histone H3K4me3 binding sites on chromosome 12.
Histone H3K4me3 antibody tested by Western blot.
The analysis was performed using 20 µg HeLa nuclear cell extract and Histone H3K4me3 antibody at 2 µg/ml.
*For optimal results, we recommend a High Salt & Sonication Protocol when preparing nuclear extracts. Visit activemotif.com to download the protocol.
Histone H3 trimethyl Lys4 antibody tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H3 trimethyl Lys4 antibody for trimethyl-Lys4 of histone H3. Peptides corresponding to regions around major sites of histone H3 methylation were spotted onto PVDF and probed with Histone H3 trimethyl Lys4 antibody at a dilution of 1 µg/ml . The amount of peptide (in picomoles) spotted is indicated next to each row. Top panel: Lane 1: unmodified Lys4. Lane 2: monomethyl Lys4. Lane 3: dimethyl Lys4. Lane 4: trimethyl Lys4. Lane 5: unmodified Lys9, 14, 18. Lane 6: monomethyl Lys9. Lane 7: dimethyl Lys9. Lane 8: trimethyl Lys9. Lane 9: dimethyl Lys14. Lane 10: monomethyl Lys18. Lane 11: dimethyl Lys18. Lane 12: trimethyl Lys18. Bottom panel: Lane 1: Unmodified Lys23. Lane 2: Monomethyl Lys23. Lane 3: Dimethyl Lys23. Lane 4: Trimethyl Lys23. Lane 5: unmodified Lys27. Lane 6: monomethyl Lys27. Lane 7: dimethyl Lys27. Lane 8: trimethyl Lys27. Lane 9: unmodified Lys36. Lane 10: monomethyl Lys36. Lane 11: dimethyl Lys36. Lane 12: trimethyl Lys36.
Detection of H3K4me3 by immunofluorescence
U2OS cells were stained with H3K4me3 antibody at a dilution of 1:500. Left panel: DAPI. Middle panel: H3K4me3 antibody staining Right panel: merge.
qPCR Primers
Active Motif's Human Positive Control Primer Set ACTB-2 (Cat. No. 71005) and Human Negative Control Primer Set 1 (Cat. No. 71001) have been validated for performing qPCR and endpoint PCR when this antibody has been used for ChIP on human samples. For ChIP experiments with this antibody on mouse samples, the Mouse Positive Control Primer Set Actb-2 (Cat. No. 71017) and Mouse Negative Control Primer Set 1 (Cat. No. 71011) have been validated for both qPCR and endpoint PCR.
Chromatin IP Assays
This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H3K4me3 antibody (pAb).
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt. This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- TIP-ChIP = Tagmented, Indexed, and Pooled Chromatin Immunoprecipitation;
- WB = Western Blotting
