Histone H3K9ac antibody (pAb)

RRID: AB_2793268
Catalog No: 39585 Format: 100 µl ¥5,670 Add to Cart
Catalog No: 39586 Format: 10 µl ¥1,430 Add to Cart

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Antibody Type:
Polyclonal
Isotype:
Serum
Purification:
None
Host:
Rabbit
Molecular Weight:
17 kDa
Reactivity:
Human, Wide Range Predicted

Applications

Western Blot Validated Immunofluorescence Validated Dot Blot Validated Immunocytochemistry Validated

Published Applications

The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:

ChIP
WB
IF

View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.

Immunogen

This Histone H3 acetyl Lys9 antibody was raised against a peptide containing acetyl-lysine 9 of human histone H3.

Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

View our Guide to Histone Modifications and Biological Function.

 
Histone H3K9ac antibody (pAb) tested by immunofluorescence.

Histone H3 acetyl Lys9 pAb tested by immunofluorescence.
Top left: HeLa cells stained with Histone H3 acetyl Lys9 pAb (1:1,000). Top right: Same cells stained with alpha Tubulin mAb (Clone 5-B-1-2). Bottom left: Stained with DAPI. Bottom right: Merge of all 3 images.

Histone H3K9ac antibody (pAb) tested by Western blot.

Histone H3 acetyl Lys9 pAb tested by Western blot.
HeLa nuclear extract (20 µg per lane) probed with Histone H3 acetyl Lys9 pAb (1:5,000).
    Lane 1: No treatment.
    Lane 2: Cells treated with sodium butyrate.

Histone H3K9ac antibody (pAb) tested by dot blot analysis.

Histone H3 acetyl Lys9 pAb tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H3 acetyl Lys9 pAb for acetyl Lys9 histone H3. Acetylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at a dilution of 1:5,000. The amount of peptide (picomoles) spotted is indicated next to each row.
    Lane 1: acetyl-Lys9 peptide.
    Lane 2: unmodified Lys9 peptide.
    Lane 3: acetyl-Lys14 peptide.
    Lane 4: unmodified Lys14 peptide.
    Lane 5: acetyl-Lys18 peptide.
    Lane 6: acetyl-Lys23 peptide.
    Lane 7: acetyl-Lys27 peptide.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • TIP-ChIP = Tagmented, Indexed, and Pooled Chromatin Immunoprecipitation;
  • WB = Western Blotting