C2C12 nuclear extract (Differentiated)

Catalog No: 36078 Format: 200 µg ¥2,990 Add to Cart

Contents

200 µg of C2C12 (Differentiated) nuclear extract at 2.72 µg/µl.

Background

C2C12 nuclear extract (Differentiated) was prepared from subconfluent undifferentiated cell cultures of the mouse myoblast C2C12 cell line. Under appropriate conditions, such as androgen stimulation, these fibroblast cells can differentiate to a skeletal muscle phenotype, forming contractile myotubes and expressing characteristic skeletal muscle proteins. When treated with bone morphogenic protein 2 (BMP-2), these cells shift differentiation pathways from myoblastic to osteoblastic and are useful in studies examining BMP-mediated Smad signaling. Due to their differentiative potential, these cells are commonly used as cell models to study skeletal muscle and bone differentiation. In addition, these cells express TGFβ receptors. Therefore, these cells are also commonly used in studies related to the TGFβ signaling pathway.

Application Notes

C2C12 nuclear extract (Differentiated) is specifically recommended for analysis of 1) myoblast differentiation, 2) osteoblast differentiation, and 3) TGFβ signaling cascades.

Extract Origin

Murine Muscle Myoblast

Extract Composition

C2C12 nuclear extract (Differentiated) is supplied in Dilution Buffer (20 mM Hepes (pH 7.9), 100 mM KCl, 1 mM MgCl2, 20% glycerol, 0.5 mM PMSF and 0.5 mM DTT).

Quality Control

Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).

Storage

To ensure stability, extracts should be stored at -80°C.

We recommend aliquoting the extracts into single-use fractions and then storing them at -80°C. This eliminates repeated freeze/thaw cycles.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.