DU 145 nuclear extract

Catalog No: 36037 Format: 200 µg ¥2,860 Buy

Contents

200 µg of DU 145 nuclear extract at 5.68 µg/µl.

Background

DU 145 nuclear extract was prepared from cell cultures of the human prostate carcinoma DU 145 cell line. The DU 145 cell line was originally derived from a brain metastasis of a male patient with prostate carcinoma and lymphocytic leukemia. In culture, these cells form epithelial-like monolayers that contain microvilli, tonofilaments and desmosomes. The DU 145 cell line, along with the PC3 cell line, are considered to be the classic prostate cancer cell lines and are most commonly used as in vitro models for prostate cancer growth and behavior. Prostate cancer is the second most common neoplasia in men. These cells exhibit moderate metastatic potential and have been demonstrated to induce adenocarcinoma in nude mice. DU 145 cells are not hormone sensitive, but do express androgen and NFκB-activating receptors, and are weakly positive for acid phosphatase.

Application Notes

DU 145 nuclear extract is specifically recommended for use in studies related to prostate cancer.

Extract Origin

Human Prostate (metastatic site: brain)

Extract Composition

DU 145 nuclear extract is supplied in Dilution Buffer (20 mM Hepes (pH 7.9), 100 mM KCl, 1 mM MgCl2, 20% glycerol, 0.5 mM PMSF and 0.5 mM DTT).

Quality Control

Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).

Storage

To ensure stability, extracts should be stored at -80°C.

We recommend aliquoting the extracts into single-use fractions and then storing them at -80°C. This eliminates repeated freeze/thaw cycles.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.