Recombinant SORD protein

Aliases: Sorbitol Dehydrogenase, L-iditol 2-dehydrogenase, SORD1
Catalog No: 81294 Format: 50 µg ¥3,600 Buy
Catalog No: 81994 Format: 1 mg ¥24,000 Buy

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Expressed In: E. coli Protein Species: Human

Contents

A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.

Background

SORD (Sorbitol Dehydrogenase), also called as RDH, SDH or XDH, is a polyol dehydrogenase that catalyzes the reversible NAD(+)-dependent oxidation of various sugar alcohols. It is mostly active with D-sorbitol (D-glucitol), L-threitol, xylitol and ribitol as substrates, leading to the C2-oxidized products D-fructose, L-erythrulose, D-xylulose, and D-ribulose, respectively. SORD is a key enzyme in the polyol pathway that interconverts glucose and fructose via sorbitol, which constitutes an important alternate route for glucose metabolism. The polyol pathway is believed to be involved in the etiology of diabetic complications, such as diabetic neuropathy and retinopathy, induced by hyperglycemia. It also may play a role in sperm motility by using sorbitol as an alternative energy source for sperm motility. Besides, SORD may have a more general function in the metabolism of secondary alcohols since it also catalyzes the stereospecific oxidation of (2R,3R)-2,3-butanediol. NADP(+) cannot be used as the electron acceptor for SORD dehydrogenase activity.

Application Notes

This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.

Protein Details

Full length SORD protein (accession number NP_003095.2) was expressed in E. coli cells with an N-terminal 6xHis Tag. The molecular weight of the protein is 41.9 kDa.

 
Recombinant SORD protein gel

Recombinant SORD protein gel
10% SDS-PAGE with Coomassie blue staining

MW: 41.9kDa

Purity: >90%

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is guaranteed for 6 months from date of arrival.