The Mitochondrial Fractionation Kit is designed to facilitate the study of apoptotic and signal transduction pathways. It provides a unique formulation of buffers and reagents that enable isolation of highly enriched mitochondrial and cytosolic fractions from various mammalian cell lines, including both apoptotic and non-apoptotic cells. The fractionation procedure is simple and easy to perform. No ultracentrifugations are required and no toxic chemicals are involved.
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|Mitochondrial Fractionation Manual|
|Mitochondrial Fractionation Profile|
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The Mitochondrial Fractionation Kit's high-quality reagents and optimized protocol eliminate cross-contamination and produce high yields of properly segregated mitochondrial & cytosolic fractions (Figure 1). Extracts are ideal for use in procedures that require mitochondrial extract, like gelshift assays, Western blot analysis, ELISA or other assays.
The Mitochondrial Fractionation Kit advantage
- Quality-controlled reagents ensure reproducibility
- No need to optimize your own procedure
- Complete kit contains all required reagents
- Prepare both the cytosolic & mitochondrial fractions
Figure 1: Localization of cytochrome c in HeLa cells.
Contents & Storage
Each Mitochondrial Fractionation Kit contains sufficient reagents to perform 100 mitochondrial and cytosolic extractions from a 100 mm tissue culture dish of cells grown to confluence.
Selection of published references for the Mitochondrial Fractionation Kit
- “Mitochondrial Effects of PGC-1alpha Silencing in MPP+ Treated Human SH-SY5Y Neuroblastoma Cells.” by Ye et al (2017) Front Mol Neurosci. 10: 164.