LightSwitch 启动子& 远端调控元件荧光素酶报告基因载体

genome-wide collections of pre-cloned luciferase reporter vectors

The LightSwitch™ collection includes over 18,000 human endogenous promoters and transcription factor response elements cloned into LightSwitch reporter vectors. These transfection-ready LightSwitch GoClones® together with the highly optimized assay reagents, controls and detailed protocols allows you to perform your gene regulation reporter assay experiments immediately. In addition, the Validated Pathway Collections, includes promoter constructs that have been validated to show significant activation or repression in response to pathway-specific induction treatments.

To ensure high-quality results, LightSwitch reporter vectors utilize a novel, engineered luciferase gene (RenSP) and optimized reagents that provide industry-leading sensitivity. For more information, please see the tabs below as well as the Overview and RenSP Luciferase sections on the Functional Genomics - LightSwitch™ page.

Click the button below to search the LightSwitch Reporter Collections and place your order on our affiliate website, SwitchGear Genomics.

Find and order LightSwitch™ GoClones®

To find LightSwitch GoClone® constructs that contain the promoters of your genes of interest, use the box below to search the LightSwitch Promoter Reporter Collection. Place your order using the specific Product ID(s) you find for the clone(s) you wish to order. As we cannot stock every GoClone® in all of our offices, delivery may take 10-14 days.   Click here for Search Tips »

IMPORTANT:  Because all LightSwitch reporter constructs contain the optimized RenSP luciferase gene, you MUST use our LightSwitch Luciferase Assay Reagents to obtain optimal results. (Other luciferase assay reagents are not compatible with RenSP.) We also recommend that you include appropriate positive & negative control vectors when you perform your assays.


Getting Started with your LightSwitch Promoter Assay

The LightSwitch Promoter Reporter Collection makes it possible to measure the functional response of promoters to stimulus in nearly any cell-based system. Plus, advances in gene assay reagent and transfection technology eliminate the need for co-transfection in most studies.

Measure promoter activity to:

  • Understand the mechanism by which a gene is induced or repressed
  • Characterize the functional consequences of transcription factor binding
  • Measure the effect of sequence variants on promoter function

Diagram depicting the luciferase activity in response to transcription factor binding to a promoter on the LightSwitch Promoter Reporter vector

Figure 1: LightSwitch assay to measure stimulated transcription factors binding to the test promoter.

Studying transcription factor activity with the LightSwitch System

Transcription factors are important regulators of eukaryotic transcriptional regulation, and they interact with DNA-encoded regulatory elements like promoters to modulate when, where and how much of a gene’s mRNA gets made. The activity of transcription factors can be affected by the cell’s environmental conditions including exposure to signaling molecules like hormones and growth factors. Reporter assays are a powerful technique for measuring the activity of promoters in living cells. LightSwitch Reporter Constructs are created by isolating a promoter from the human genome and cloning it upstream of the RenSP luciferase reporter gene on a plasmid. Once this plasmid is transfected into a living cell, a change in promoter activity causes a change in the reporter signal (light output).

Graph showing dose response of a LightSwitch SYT8 Promoter Reporter vector stimulated by 5 different estrogen compounds

Figure 2: Response of LightSwitch SYT8 Promoter Reporter vector to various estrogen compounds.

Dose response data is shown for the SYT8 promoter (Product ID S714388) for five different estrogenic compounds. The graph summarizes the luciferase activity of this promoter in HT1080 human fibrosarcoma cells that were co-transfected with an ER cDNA expression plasmid and then treated with various concentrations of estrogen compounds for 24 hours before the luminescence was measured using the LightSwitch Luciferase Assay Kit. The Y-axis shows the relative luminescent values while the X-axis shows the molar concentration of compound on a log10 scale. The Coefficients of Variance (CVs) ranged from 10% to 12%.

The LightSwitch Luciferase Assay System and Promoter Reporter Collection are important tools for determining how a transcription factor regulates its target genes. Researchers can monitor changes in any promoter’s activity in response to variations in transcription factor function that may be modulated by techniques like over-expression (often by cDNA co-transfection; Figure 2), knock-down (often by siRNA co-transfection) or treatment with a known agonist or antagonist. LightSwitch Promoter Reporter constructs are also useful for assessing the functional consequences of transcription factor binding detected through a ChIP-based experiment and for measuring the effects of mutagenizing a putative transcription factor binding motif.


  • Monitoring changes in promoter activity in response to variations in transcription factor function.
  • Assessing the functional consequences of transcription factor binding detected through a ChIP-based experiment.
  • Measuring the effects of mutagenizing a putative transcription factor binding motif.
  • Verifying computational predictions and supplementing microarray or Next-Generation sequencing data.

Our scientists created the LightSwitch Promoter Reporter Collection by using an algorithm to evaluate over 5 million human cDNA sequences in order to identify transcription start sites (TSS) throughout the human genome. Based on these TSS predictions, over 18,000 human promoter sequences of ~1 kb (-900…+100 bp relative to the TSS) were cloned into the pLightSwitch_Prom reporter vector, which utilizes the novel, engineered RenSP luciferase gene. After transfection, luciferase activity is assayed using the optimized regents found in LightSwitch Luciferase Assay Kit.

In addition to the over 18K endogenous promoter clones that make up the LightSwitch Promoter Reporter Collection, Active Motif also offers a collection of LightSwitch Synthetic Response Elements. These constructs contain repeats of a transcription factor binding site motif, cloned upstream of a minimal HSV-TK promoter and the RenSP luciferase gene in the Long-range Enhancer Reporter Vector, pLightSwitch_LR vector. This makes them ideal for use as a primary screen to identify compounds or conditions that affect the activity of a biological pathway. Afterward, a secondary screen can be performed using related endogenous promoter reporter constructs to detect subtle differences between compounds or conditions.

The following publications cite the use of and/or provide additional information about the LightSwitch Luciferase Assay System: