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Recombinant EPHB4 (561-987) protein
| Aliases: Ephrin type-B receptor 4, TYRO11, HTK, MYK1, Tyrosine-Protein Kinase Receptor HTK |
| Catalog No: 81446 | Format: 20 µg | ¥4,000 | Add to Cart |
| Catalog No: 81546 | Format: 1 mg | ¥32,000 | Add to Cart |
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Expressed In: Baculovirus Protein Species: Human
Contents
A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.
Background
EPHB4(Ephrin type-B receptor 4) is a Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Together with its cognate ligand/ functional ligand EFNB2 it is involved in the regulation of cell adhesion and migration, and plays a central role in heart morphogenesis, angiogenesis and blood vessel remodeling and permeability. EPHB4-mediated forward signaling controls cellular repulsion and segregation from EFNB2-expressing cells.
Application Notes
This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.
Protein Details
Recombinant EPHB4 (561-987) protein that includes amino acids 561-987 of human EPHB4 protein (accession number NP_004435.3) was expressed in a baculovirus expression system, and contains an N-terminal FLAG tag. The molecular weight of the protein is 48.89 kDa.
References
Human
Recombinant EPHB4 (561-987) protein
10% SDS-PAGE Coomassie staining
MW: 48.89 kDa
Purity: ≥ 95%
HTRF assay for EPHB4 (561-987) activity
1 μM TK substrate was incubated with different concentrations of EPHB4 (564-987) protein in 10 μl reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, 1 mM MnCl2, 5 nM SEB and 100 μM ATP for 1 hour. Then 10 μl detection reagents containing anti-TK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.
Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee
This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.
