Recombinant GSK3β protein
Aliases: Glycogen Synthase Kinase 3 Beta, GSK-3 Beta |
Catalog No: 31173 | Format: 10 µg | Discontinued |
Expressed In: Baculovirus Protein Species: Human
Contents
A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.
Application Notes
Recombinant GSK3β is suitable for kinase assays and Western blot. The molecular weight of the protein is ~76.312 kDa. The activity of the protein is ~ 2 pmol/µg min. Recommended kinase reaction conditions: 60 mM HEPES-NaOH, pH 7.5, 3 mM MgCl2, 3 mM MnCl2, 3 µM Na-orthovanadate, 1.2 mM DTT, ATP (variable), 2.5 µg/50 µl PEG20.000, Substrate: Rb-CTF 5 µg/50 µl, Recombinant GSK3β: 200 ng/50 µl. Kinase activity may vary depending on the substrate and reaction conditions used.
Protein Details
Recombinant human GSK3β protein was produced using baculovirus infected Sf9 cells. The protein was made against amino acids M1-T420 , accession number L33801 and N-terminally fused to a GST-HIS6-Thrombin cleavage site. Purified by GSH-agarose affinity purification.
Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.