Recombinant UBE2F protein

Aliases: Ubiquitin Conjugating Enzyme E2 F, NEDD8 conjugating enzyme, NCE2
Catalog No: 81484 Format: 100 µg ¥3,680 Buy
Catalog No: 81584 Format: 1 mg ¥22,680 Buy

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Expressed In: E. coli Protein Species: Human

Contents

A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.

Background

UBE2F (NEDD8-conjugating enzyme UBE2F) also known as NCE2. UBE2F is a member of the E2 ubiquitin-conjugating enzyme family which is required for post-replicative DNA damage repair and plays an important role in various cellular processes. UBE2F can accept the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5.

Application Notes

This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product.  All available data this product is shown. 

Protein Details

Recombinant UBE2F protein that includes full length of human UBE2F protein (accession number NP_001265234.1) was expressed in E. coli and contains an N-terminal His tag with a molecular weight of 23.24 kDa. The purity of the protein is ≥ 95% by SDS-PAGE.

References

Human

 
Recombinant UBE2F protein

Recombinant UBE2F protein
12.5% SDS-PAGE Coomassie staining
MW: 23.24 kDa kDa
Purity: ≥ 95%

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.