Recombinant UBE2I protein

Aliases: Ubiquitin conjugating enzyme E2 I, SUMO conjugating enzyme UBC9, C358B7.1, P18, UBC9, ubiquitin carrier protein 9, SUMO protein ligase, P18
Catalog No: 81489 Format: 100 µg ¥3,680 Buy
Catalog No: 81589 Format: 1 mg ¥22,680 Buy

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Expressed In: E. coli Protein Species: Human

Contents

A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.

Background

UBE2I (SUMO-conjugating enzyme UBC9) also known as C358B7.1, P18, UBC9. UBE2I is a member of the E2 ubiquitin-conjugating enzyme family which is required for post-replicative DNA damage repair and plays an important role in various cellular processes. UBE2I can accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3, SUMO4 and SUMO1P1/SUMO5 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2, CBX4 and ZNF451. Can catalyze the formation of poly-SUMO chains. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation. Sumoylates p53/TP53 at ‘Lys-386’. Mediates sumoylation of ERCC6 which is essential for its transcription-coupled nucleotide excision repair activity.

Application Notes

This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product.  All available data this product is shown. 

Protein Details

Recombinant UBE2I protein that includes full length of human UBE2I protein (accession number NP_003336.1) was expressed in E. coli and contains an N-terminal His tag with a molecular weight of 20.17 kDa. The purity of the protein is ≥ 95% by SDS-PAGE.

References

Human

 
Recombinant UBE2I protein

Recombinant UBE2F protein
12.5% SDS-PAGE Coomassie staining
MW: 20.17 kDa kDa
Purity: ≥ 95%

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.