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Recombinant SETD6 protein
| Aliases: SET Domain Containing 6, Protein Lysine Methyltransferase, N-Lysine Methyltransferase SETD6, EC 2.1.1 |
| Catalog No: 81282 | Format: 20 µg | ¥3,720 | Buy |
| Catalog No: 81982 | Format: 1 mg | ¥32,000 | Buy |
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Expressed In: Baculovirus Protein Species: Human
Contents
A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.
Background
SETD6 (SET Domain Containing 6, Protein Lysine Methyltransferase) is a member of SETD family, which has methyltransferase activity. This protein was reported to monomethylate ‘Lys-8’ of histone H2AZ, which is involved in nuclear receptor-dependent transcription. SETD6 was also reported to monomethylates ‘Lys-310’ of the RELA subunit of NF kappa-B complex, leading to down-regulate NF-kappa-B transcription factor activity. It may interact with several endogenous proteins which are involved in nuclear hormone receptor signaling. However, the real substrates remain unknown for a very long time.
Application Notes
Recombinant SETD6 protein is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.
Protein Details
Full length human SETD6 protein (accession number NP_079136.2) was expressed in a baculovirus expression system and contains an N-terminal FLAG tag. The molecular weight of SETD6 is 52.1 kDa.
Recombinant SETD6 protein gel
10% SDS-PAGE with Coomassie staining.
MW: 52.1 kDa
Purity: >90%
MTase-Glo Assay for Recombinant SETD6 protein methyltransferase activity
1 µM substrate peptide and 1 µM SAM were incubated with different concentrations of recombinant SETD6 protein in an 8 µl reaction system containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP for 1 hr. 5×MTase-Glo Reagent was added to the products and incubated for 30 min. Then MTase-Glo Detection was added and luminescence was read after another 30 min incubation. SAH standard curve (0-1 µM) was performed following the same protocol
MTase-Glo Assay for Recombinant SETD6 protein methyltransferase activity
1 µM substrate peptide and 1 µM SAM were incubated with different concentrations of recombinant SETD6 protein in an 8 µl reaction system containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP for 1 hr. 5×MTase-Glo Reagent was added to the products and incubated for 30 min. Then MTase-Glo Detection was added and luminescence was read after another 30 min incubation. SAH standard curve (0-1 µM) was performed following the same protocol
Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee
This product is guaranteed for 6 months from date of arrival.
