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Biotin-14-dATP
| Catalog No: 14138 | Format: 1 mg | ¥17,160 | Buy |
| Catalog No: 14139 | Format: 200 µg | ¥4,230 | Buy |
| Application: Hi-C |
Description
Biotin-14-dATP at 1 mg/mL (1mM) in 10 mM Tris, pH 7.5, 0.1 mM EDTA. Suitable for direct enzymatic labeling of DNA.
Storage Conditions
Store solution at -20°C.
This product is guaranteed for 12 months from date of receipt. This product is for research use only and is not for use in diagnostic procedures.
References
Selected References:
de Wit et al. (2012) A decade of 3C technologies: insights into nuclear organization. Genes Dev. 26 (1):11.
Lieberman-Aiden et al. (2009) Comprehensive mapping of long range interactions reveals folding principles of the human genome. Science 326 (5959):289.
Nagano et al. (2015) Single-cell Hi-C for genome-wide detection of chromatin interactions that occur simultaneously in a single cell. Nature Protocols 10 (12):1987.
Belaghzal et al. (2017) Hi-C 2.0: An optimized Hi-C procedure for high-resolution genome-wide mapping of chromosome confirmation. Methods 123:56.
Berkum et al. (2010) Hi-C: A Method to Study the Three-dimensional Architecture of Genomes. J. Vis. Exp. 39:1869.
Structure of Biotin-14-dATP
DNA polymerase incorporation of biotin-14-dATP via nick translation.
pBR322 DNA modified via a Pol I/DNAse I nick translation assay with a dNTP mix containing Active Motif’s biotin-14-dATP as the ATP source. The reaction was terminated, and the DNA was spin column purified, quantified and spotted on nitrocellulose membrane. Biotin incorporation was assessed by incubating the membrane with HRP-conjugated anti-biotin antibody. A, nick translation with ATP. B and C, nick translation with biotin-14-ATP (biological replicates).
Hi-C Contact Frequency vs. Genomic Distance
Similar Distance-Dependence of Contact Frequency. Hi-C datasets generated using Active Motif’s biotin-14-dATP and another commercial reagent (Rao ref) show a similar distribution of contacts ranging from short- to long-distance, indicating that biotin-based capture using the Active Motif reagent is unbiased.
