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Recombinant DAPK3 / ZIPK protein
| Aliases: Death Associated Protein Kinase 3, DAP Kinase 3, ZIPK, Ziper interacting protein kinase |
| Catalog No: 81432 | Format: 20 µg | ¥3,720 | Buy |
| Catalog No: 81532 | Format: 1 mg | ¥32,000 | Buy |
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Expressed In: Baculovirus Protein Species: Human
Contents
A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.
Background
DAPK3 (Death Associated Protein Kinase 3), a serine/threonine kinase which is involved in the regulation of apoptosis, autophagy, transcription, translation and actin cytoskeleton reorganization. This protein is involved in the regulation of smooth muscle contraction and regulates both type I (caspase-dependent) apoptotic and type II (caspaseindependent) autophagic cell deaths signal, depending on the cellular setting. DAPK3 is involved in regulation of starvation-induced autophagy and regulates myosin phosphorylation in both smooth muscle and non-muscle cells. In smooth muscle, DAPK3 regulates myosin either directly by phosphorylating MYL12B and MYL9 or through inhibition of smooth muscle myosin phosphatase (SMPP1M) via phosphorylation of PPP1R12A; the inhibition of SMPP1M functions to enhance muscle responsiveness to Ca(2+) and promote a contractile state. DAPK3 phosphorylates MYL12B in non-muscle cells leading to reorganization of actin cytoskeleton. Isoform 2 can phosphorylate myosin, PPP1R12A and MYL12B.
Application Notes
Recombinant DAPK3 / ZIPK protein is suitable for use in in enzyme kinetics, inhibitor screening, and selectivity profiling.
Protein Details
Recombinant DAPK3 / ZIPK protein was expressed in baculovirus expression system as the full length protein (accession number NP_001339.1) with a N-terminal FLAG tag. The molecular weight of the protein is 53.8 kDa.
References
Human
Recombinant PRKCE; protein
10% SDS-PAGE Coomassie staining
MW: 53.8 kDa
Purity: ≥ 90%
HTRF assay for DAPK3 / ZIPK; activity
1 µM STK S1 substrate was incubated with different concentrations of DAPK3 / ZIPK; protein in a 10 µl reaction system containing 1×Enzymatic Buffer, 10 mM MgCl2, 1 mM DTT and 100 µM ATP for 1 hour. The detection reagents were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature.
HTRF assay for DAPK3 / ZIPK; activity
1 µM STK S1 substrate was incubated with different concentrations of DAPK3 / ZIPK; protein in a 10 µl reaction system containing 1×Enzymatic Buffer, 10 mM MgCl2, 1 mM DTT and 100 µM ATP for 1 hour. The detection reagents were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature.
Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee
This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.
