Recombinant CENPB protein

Aliases: Centromere Protein B, Major Centromere Autoantigen B
Catalog No: 81201 Format: 100 µg ¥3,600 Buy
Catalog No: 81901 Format: 1 mg ¥24,000 Buy

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Expressed In: E. coli Protein Species: Human

Contents

A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.

Background

CENPB (Centromere Protein B), also called as CENP-B, is a highly conserved protein that facilitates centromere formation. It is a DNA-binding protein that is derived from transposases of the pogo DNA transposon family. It contains a helix-loop-helix DNA binding motif at the N-terminus, and a dimerization domain at the C-terminus. CENPB can interact with centromeric heterochromatin in chromosomes and binds to a specific 17 bp subset of alphoid satellite DNA, called the CENP-B box. It may organize arrays of centromere satellite DNA into a higher-order structure which then directs centromere formation and kinetochore assembly in mammalian chromosomes. CENPB is proposed to play an important role in the assembly of specific centromere structures in interphase nuclei and on mitotic chromosomes. It is also considered a major centromere autoantigen recognized by sera from patients with anti-centromere antibodies.

Application Notes

This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product.  All available data this product is shown.

Protein Details

Recombinant human CENPB protein was expressed in E.coli cells as the full length protein (accession number NP_001801) with an N-terminal 6xHis Tag. The molecular weight of the protein is 68.7 kDa.

 
Recombinant CENPB proteingel

Recombinant CENPB protein gel
10% SDS-PAGE Coomassie staining
MW: 68.7 kDa
Purity: >85%

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.