CtIP antibody (mAb)

RRID: AB_2714164
Clone: 14-1
Aliases: RBBP8, RIM, COM1, JWDS, SAE2, SCKL2
Catalog No: 61141 Format: 100 µg ¥5,500 Buy
Catalog No: 61142 Format: 10 µg ¥1,380 Buy
Catalog No: 61942 Format: 50 µg ¥3,140 Buy

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Antibody Type:
Monoclonal
Isotype:
IgG1
Purification:
Protein A Chromatography
Host:
Mouse
Molecular Weight:
120 kDa
Reactivity:
Human

Applications

ChIP Validated Western Blot Validated Immunoprecipitation Validated Immunofluorescence Validated Immunocytochemistry Validated

Application Notes

Applications Validated by Active Motif:
WB: 1 - 2 µg/ml

Published Applications

The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:

IP
ChIP
ICC/IF
WB

View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.

Immunogen

This CtIP antibody was raised against a recombinant protein corresponding to amino acid residues 620-897 of human CtIP.

Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

References

CtIP antibody (mAb) - 100 µg (Cat. No. 61141)

 
 
 

 

CtIP antibody mpAb) tested by Western blot
20 µg of HEK293 nuclear extract was run on SDS-PAGE and probed with antibody at 1 µg/ml.

Background

CtIP (CTBP interacting protein, RBBP8, RIM, COM1) is a transcriptional regulator and tumor suppressor first identified through its interaction with CTBP (C-Terminal Binding Protein). CtIP binds to the Rb protein and serves as a transcriptional repressor, through recruitment of CtBP and also by interaction with general transcription factors TFIID and TFIIB. CtIP also interacts with Rb-related protein p130 and Ikaros, a master regulator in lymphocyte development. CtIP is phosphorylated upon DNA damage (likely by ATM and/or ATR), interacts with BRCA1 and plays a critical role in facilitating DNA damage repair. Mutations that abolish the interaction between CtIP and BRCA1 result in loss of cell cycle control leading to the development of cancer.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with CtIP antibody (mAb).

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting