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TBK1 antibody (mAb)
| RRID: AB_2793432 |
| Clone: 108A429 |
| Aliases: NAK, T2K |
| Catalog No: 40909 | Format: 100 µg | ¥5,500 | Buy |
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Applications
Application Notes
Applications Validated by Active Motif:
WB: 1 - 2 µg/ml dilution
For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all blocking solutions may also reduce background. Individual optimization may be required.
Immunogen
This TBK1 antibody was raised against a peptide corresponding to amino acid residues 563-577 of human TBK1.
Buffer
PBS containing 0.02% sodium azide. Sodium azide is highly toxic.
TBK1 mAb tested by Western blot.
TBK1 detection by Western blot. The analysis of TBK1 was performed using TBK1 mAb at a 2 µg/ml dilution and nuclear extract from 293 cells (lane 1) and nuclear extract from 293 cells that had been transfected with human TBK1 cDNA (lane 2).
Background
TBK1 – TANK-binding kinase 1 is a regulator of the NFκB (NFκB p50 & NFκB p65) signaling pathway. NFκB signaling is controlled to a large extent by the sequestration of the NFκB complex in the cytoplasm by its association with one of the IκB family of proteins. IκB is phosphorylated by the IkB Kinase (IKK) complex, resulting in the degradation of IκB and the nuclear translocation of NFκB. TANK is an IκB family member that is specifically recognized and phosphorylated by TBK1, leading to the destruction of TANK and the activation of the NFκB pathway.
Positive Control
Active Motif's 293 nuclear extract (Catalog No. 36033) can be used as a positive control for TBK1 antibody.
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Store at 4°C for short term. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- WB = Western Blotting
