GCN5 antibody (mAb)

RRID: AB_2793415
Clone: 2GC-2C11
Aliases: KAT2A, hGCN5, GCN5L2, PCAF-b
Catalog No: 39975 Format: 100 µg Discontinued

Antibody Type:
Monoclonal
Isotype:
IgG1
Purification:
Protein A Chromatography
Host:
Mouse
Molecular Weight:
95 kDa
Reactivity:
Human

Applications

Western Blot Validated Immunofluorescence Validated Immunocytochemistry Validated

Application Notes


This product has been discontinued:
Please refer to our alternative product Catalog No. 39831


Published Applications:
ICC/IF
WB
See references for more information. Individual optimization may be required.

Immunogen

This GCN5 antibody was raised against a synthetic peptide corresponding to amino acids 118-132 of human GCN5.

Buffer

PBS pH 7.5 containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

Background

GCN5 (General control of nutrition 5, KAT2A) is a histone acetyltransferase (HAT) and serves as a transcriptional activator. GCN5 is a component of the SAGA and ATAC complexes, which have histone acetyltransferase activity for histones H3 and H4. GCN5 is required for neural tube closure and embryonic development.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting